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:2* Taq PCR master mix with blue dye

Description
This Taq PCR Master mix contains Taq DNA Polymerase, dNTPs, and optimized buffer and the blue dye. This Taq Mastermix is provided at 2× concentration and used at 1× concentration by adding template, primer, and H2O. It has the 5′to 3′DNA polymerase activity and 5′to 3′exonuclease activity without 3′-5′exonuclease activity.The extending speed is 1-2 kb/min.There is an “A” on 3′end.The PCR product can be cloned in TA vector.

Quality Control:
This product has passed the following quality control assays: functional absence of double- and single-stranded endonuclease activity; >90% homogeneous by SDS gel electrophoresis.Each lot of Taq DNA Polymerase is assayed for amplification from as little as 10 ng of human genomic DNA.

Application:
 

  • PCR
  • Primer Extension
  • Microarray Analysis
  • High-Throughput PCR

Storage: Store at -20°C
Notes:
no repeated freezing and thawing and split charging for good amplification.

 

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