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Products:2xTaq PCR MasterMix(dye-) supplied by bulk package
Description of the 2* PCR master mix:
This PCR master mix contains the Taq DNA Polymerase, dNTPs, and optimized buffer but without any dye in it. This Taq Mastermix is provided at 2× concentration and used at 1× concentration by adding template, primer, and H2O. It has the 5′to 3′DNA polymerase activity and 5′to 3′exonuclease activity without 3′-5′exonuclease activity.The extending speed is 1-2 kb/min.There is an "A" on 3′end.The PCR product can be cloned in TA vector.
Quality Control:
This product has passed the following quality control assays: functional absence of double- and single-stranded endonuclease activity; >90% homogeneous by SDS gel electrophoresis.Each lot of our Taq DNA Polymerase is assayed for amplification from as little as 10 ng of human genomic DNA
Application:
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PCR
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Primer Extension
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Microarray Analysis
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High-Throughput PCR
Storage: Store at -20°C
Shipping with blue ice
Reaction Mixture Set Up
Template <0.5 μg
Forward Primer (10 μM) 1 μl
Reverse Primer (10 μM) 1 μl
2×TransTaq PCR SuperMix 25 μl
ddH2O to final volume 50 μl
No repeated freezing and thawing and split charging for good amplification.
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