:2×Taq PCR MasterMix(dye+) |
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2×Taq PCR MasterMix(dye+) Description: It contains Taq DNA Polymerase, dNTPs, and optimized buffer. This Taq Mastermix is provided at 2× concentration and used at 1× concentration by adding template, primer, and H2O. Application: PCR Primer, Extension,Microarray Analysis,High-Throughput PCR Storage: Store at -20°C Reaction Mixture Set Up Template <0.5 μg Forward Primer (10 μM) 1 μl Reverse Primer (10 μM) 1 μl 2×TransTaq PCR SuperMix 25 μl ddH2O to final volume 50 μl Recommended thermal cycling conditions 94℃ 2-5 min 94℃ 30 sec 50-60℃ 30 sec 30-35 cycles 72℃ 1 min/1-2 kb 72℃ 5-10 min Notes: no repeated freezing and thawing and split charging for good amplification. |
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