Product Number: PSE109

Shipping and Storage

1. Storage temperature: -20±5℃ for storage
2. Validity period: 2 years.

Component

Description

N-glycosidase F (PNGase F) is an effective amidase produced by recombinant Escherichia coli with cloned genes encoding the enzyme. It can cleave the N-sugar chain on glycoproteins, helping to generate carbohydrate free peptides and oligosaccharides with di-N-acetyl chitosan units. N-glycosidase F is the most effective method for removing almost all N-sugar chains from glycoproteins. The product can remove all complex, heterozygous, and high mannose sugar chains from antibodies and their fusion proteins, but if there are alpha 1,3-linked fucose residues in the core structure (often expressed in plant and insect cells as immunoglobulins), they cannot be cleaved. Obtaining accurate N-sugar chain distribution in the shortest possible time is crucial for effective process control. N-glycosidase F is an optimized reagent that can rapidly deglycosylate antibodies and fusion proteins within a few minutes. All N-sugar chains can be released quickly and without preference, and can be directly subjected to downstream chromatography or mass spectrometry analysis to quickly determine the glycosylation of antibodies.

Active definition

Unit enzyme activity is defined as the amount of enzyme required to remove over 95% of sugar chains from 10μg denatured RNase B within 1 hour in a 10μL reaction system at 37℃.

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Product Number: RNK7201

Shipping and Storage

1. This reagent kit is transported and stored at room temperature.
2. To avoid the volatilization, oxidation, and pH changes of reagents exposed to air for a long time, the lid of each solution should be promptly closed after use.

Components

Note: This reagent kit can be stored at room temperature for 12 months without affecting its effectiveness.

Description

This reagent kit uses a centrifugal adsorption column with a specially designed silica matrix membrane. The difference in adsorption capacity between columns is minimal, and the reproducibility is good. Under high salt conditions, RNA efficiently and specifically binds to silica gel adsorption membranes, while maximizing the removal of proteins, inorganic salt ions, and many organic impurities. Under low salt conditions, RNA is eluted. This product adopts a specially designed ultra micro centrifuge column, which can recover trace and ultra micro samples. The general amount of RNA samples that can be processed is 0.1-10μg. This kit is used for purifying and recovering RNA from enzyme reaction solutions (such as DNase treatment, protease treatment, RNA labeling, etc.), and can also be used for purifying RNA extracted from other methods. The purified total RNA has no protein contamination, and the obtained RNA can be used for Northern blot, Dot blot, mRNA extraction, cDNA synthesis, primer extension, differential display, etc.

Application

Suitable for rapid extraction of plant microRNA or separate extraction of microRNA/total RNA.

Features

1. The special gasket free centrifugal column design ensures no liquid residue or contamination after centrifugation. Ensuring high purity of recovered RNA.
2. The special ultra micro centrifuge column design reduces the adsorption membrane area by several times, minimizing the loss of adhesion to the adsorption membrane during recovery. At the same time, it can elute with a minimum volume of 6μL, ensuring high concentration of RNA extraction.
3. The ultra micro centrifuge column is different from other manufacturers in that it adopts our unique hydrophobic and non nucleic acid adsorbing pad base and silicone membrane design. The added base can withstand high-speed centrifugation up to 20000 revolutions per minute without causing the silicone membrane to fall off during high-speed centrifugation. High speed centrifugation ensures the removal of impurities, salt ions, and ethanol, maximizing purity.

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Product Number: RNK6901

Shipping and Storage

1. Inappropriate storage at low temperatures (4℃ or -20℃) may cause solution precipitation, affecting the effectiveness of use. Therefore, transportation and storage are carried out at room temperature (15℃-25℃).
2. To avoid the volatilization, oxidation, and pH changes of reagents exposed to air for a long time, the lid of each solution should be promptly closed after use.

Components

Note: This reagent kit can be stored at room temperature for 12 months without affecting its effectiveness.

Description

This product is suitable for rapid extraction of RNA from common plants, common polysaccharide polyphenol plants, and fungi. The exclusive development of genomic DNA clearance/RNA adsorption universal column technology combined with special reagent formula generally does not require DNA enzyme digestion, effectively removing gDNA residue, and obtaining RNA without significant DNA residue. It can be directly used for downstream reverse transcription fluorescence quantitative PCR or high-throughput sequencing library construction experiments.

Application

Suitable for rapid extraction of RNA from common plants, common polysaccharide and polyphenol plants, fungi, and other sources.

Features

1. Completely avoid using toxic reagents such as phenol, chloroform, and beta mercaptoethanol, and do not require steps such as ethanol precipitation.
2. Simple and straightforward, a single sample operation can generally be completed within 12 minutes.
3. Exclusive research and development of genomic DNA clearance/RNA adsorption universal column technology can effectively remove gDNA residues, resulting in extremely high RNA purity, with a typical OD260/OD280 ratio of 2.1~2.2. Generally, DNase digestion is not required and can be used for experiments such as reverse transcription PCR, fluorescence quantitative PCR, high-throughput sequencing library construction, etc.
4. The world's leading adaptability is extremely extensive, successfully extracting hundreds of samples including cotton, roses, Arabidopsis, rice, tobacco, poplar, etc., including some samples that failed to be extracted by domestic and foreign reagent kits. If the effect of particularly complex polysaccharide polyphenols and other samples is not satisfactory, or if other companies fail to produce particularly difficult samples, EASYspin Plus Complex Polysaccharide Polyphenols Plant RNA Extraction Kit can be selected.

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Product Number: RNK5201

Shipping and Storage

1. Improper storage of room temperature components at low temperatures (4℃ or-20℃) can cause solution precipitation and affect the effectiveness of use. Therefore, transportation and storage are carried out at room temperature (15℃ -25℃).
2. To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.

Components

Description

The unique Buffer RPA rapidly cleaves cells and inactivates cellular RNA enzymes. After adjusting the binding conditions with ethanol, RNA selectively adsorbs onto the silica matrix membrane in a highly dissociated salt state. DNase directly digests residual DNA on the column, and then through a series of rapid rinsing centrifugation steps, Buffer RW1 and Buffer RW remove impurities such as cell metabolites and proteins. Finally, low salt RNase free H₂O washes pure RNA off the silica matrix membrane.

Features

1. Completely do not use toxic substances β-Mercaptoethanol/phenol/chloroform does not require steps such as ethanol precipitation.
2. Simplicity, single sample operation can generally be completed within 40 minutes, making it the simplest and fastest reagent kit in the world.
3. The RNA obtained through DNase I column digestion without residual DNase can be directly used for reverse transcription fluorescence quantitative PCR, second-generation sequencing, chip, RACE and other experiments.
4. World leading, it is the most widely adaptable reagent kit among similar products, which can extract plants including rice, corn, wheat, Arabidopsis, tomato, tobacco, and general polysaccharides and polyphenols such as cotton and holly.
5. Multiple column washes ensure high purity, with a typical OD260/OD280 ratio of 2.0-2.2 and no DNA residue. It can be directly used for fluorescence quantitative PCR, RT-PCR, chips, second-generation sequencing, Northern blot and other experiments.

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Product Number: RDK104

Shipping and Storage

Under specified storage conditions for 2 years, please refer to the reagent kit label for details.

Components

Note: This kit does not contain ROX reference dye. If your instrument requires the addition of ROX reference dye, it needs to be purchased separately.

Description

The CHO Host Cell Residual DNA Detection Kit is a specialized reagent kit designed for the quantitative analysis of CHO host cell residual DNA content in various biological products, including intermediates, semi-finished products, and finished goods. This kit employs the probe-based fluorescence quantitative PCR principle to accurately measure the residual DNA content in samples. It features high specificity, rapid detection, stable performance, and a minimum detection limit capable of reaching the fg level.

The CHO DNA quantitative reference material included in this kit is traceable to national standard reference materials, enabling accurate quantification of CHO residual DNA in samples.

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Product Number: Q9600

Description

Q9600 is a high-throughput real-time PCR instrument developed by our company. It adheres to the principles of high efficiency andquality, combined with the advanced temperature control system andoptical system, as well as powerful software analysis functions. It canrealize applications such as qualitative/quantitative analysis, genotyping,allele identification, HRM, melting curve analysis and etc. The productadopts side scan technology and all channels are collected at the sametime. 96 samples can be scanned within 5 seconds to save the test time.This product has powerful software and hardware functions to meetthe different needs.

Features

1. 12.1" retractable color touch screen, the screen angle can be adjusted.
2. Configure automation hot lid, can be used with automation workstation, improve work efficiency.
3. Unique side scan technology, all channels can be detected simultaneously, complete the scan of all fluorescent channels of 96 samples within 5S.
4. LED light source has the advantage of energy saving, environmental protection, long service life and maintenance free.
5. Powerful software analys is functions can perform qualitative/quantitative analysis, high -resolution melting curve, genetic typing, relative quantitative, temperature gradient function, etc.

Application

1. Scientific Research: Research institutions and molecular clones,gene expression, and genotyping of various colleges and universities.
2. Clinical Diagnosis: Medical institutions perform pathogen testing, genetic screening, various infectious diseases, tumor diagnosis, etc.
3. Animal husbandry agriculture: Environmental testing, pesticide residues and soil testing.
4. Pet medica: Detectives of various pet pathogens(such as avian influenza, foot-and-mouth disease, swine fever, etc.) and health monitoring.
5. Food Safety: All kinds of food microbial detection, allergens, genetically modified food, real and false meat identification, etc.
6. In vitro diagnosis: Reagent development, verification, regeneration drugs, etc.

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Product Number: PCRD002

Packing

Description

 PCRD, also known as Nucleic Acid Lateral Flow (NALF), is a technology that uses immunochromatography to rapidly and visually detect effectively amplified DNA. When combined with technologies such as RPA and LAMP, it enables convenient nucleic acid testing without the need for specialized laboratory equipment, significantly expanding the range of applications for nucleic acid testing. PCRD002 lateral flow Cassette Used for detecting two genes (or two loci). This test strip is suitable for use with biotin FITC/FAM、 The detection of nucleic acid amplification products with third markers is suitable for PCR amplification, isothermal amplification (RPA, LAMP, etc.).

Through optimization of the reaction system, the PCRD002 kit eliminates the need for a loading buffer. Amplified nucleic acid samples can be diluted directly with deionized water and loaded for testing. Since testing can be performed without a loading buffer, this significantly improves the convenience of product shipping and the ease of use for downstream applications.

Detection Principle

This product uses colloidal gold lateral chromatography double antibody sandwich method to rapidly detect nucleic acid amplification products. The detected nucleic acid amplification product must be labeled with biotin, FITC/FAM, or a third marker at the same time. When the double labeled amplification product flows through the test strip, it can be recognized by colloidal gold labeled antibodies and nitrocellulose membrane immobilized antibodies, forming a red band in the corresponding area.

Features

1. All-solid format—no liquids—for easy transport
2. Safe to use—eliminates the need for dye loading and UV irradiation
3. Compatible with multiple amplification formats
4. No special reagents or equipment required
5. Flexible operation—nucleic acid testing can be performed anytime, anywhere
6. Results available in as little as 10 minutes
7. Higher sensitivity than gel electrophoresis
8. Capable of testing multiple strips simultaneously
9. Stable at room temperature for up to two years

Relate

PCRD001, PCRD lateral flow strips, Single target

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Product Number: PCRD001

Component

Description

PCRD, also known as Nucleic Acid Lateral Flow (NALF), is a technology that uses immunochromatography to rapidly and visually detect effectively amplified DNA. When combined with technologies such as RPA and LAMP, it enables convenient nucleic acid testing without the need for specialized laboratory equipment, significantly expanding the range of applications for nucleic acid testing.

This product uses colloidal gold lateral chromatography double antibody sandwich method to rapidly detect nucleic acid amplification products. The detected nucleic acid amplification product must be labeled with both biotin and FITC/FAM. When the double labeled amplification product flows through the test strip, it can be recognized by colloidal gold labeled antibodies and nitrocellulose membrane immobilized antibodies, forming a red band in the corresponding area.

Application

Used for single gene (or single locus) detection. This test strip is suitable for detecting nucleic acid amplification products containing biotin and FITC/FAM, and is suitable for PCR amplification, isothermal amplification (RPA, LAMP, etc.).

Features

1. All-solid format—no liquids—for easy transport
2. Safe to use—eliminates the need for dye loading and UV irradiation
3. Compatible with multiple amplification formats
4. No special reagents or equipment required
5. Flexible operation—nucleic acid testing can be performed anytime, anywhere
6. Results available in as little as 10 minutes
7. Higher sensitivity than gel electrophoresis
8. Capable of testing multiple strips simultaneously
9. Stable at room temperature for up to two years

Structural diagram

Relate

PCRD002, PCRD lateral flow strips, two target

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Product Number: ELK059

Shipping and Storage

1. Reagent kit storage: 2-8℃.
2. Validity period: 6 months.

Component

If you need to purchase universal components separately, please provide the corresponding component names.

Description

The reagent kit adopts the principle of double antibody sandwich method: the capture antibody is wrapped on the enzyme-linked immunosorbent assay (ELISA) plate, and the analyte in the sample and calibration sample is captured. The enzyme-linked secondary antibody labeled with horseradish peroxidase is added to form an "antibody antigen antibody HRP" immune complex. After adding TMB for color development, if the analyte in the sample appears blue, the reaction is stopped by adding termination solution. During the detection process, free components are washed away, and the OD value is measured at 450 nm using an enzyme-linked immunosorbent assay (ELISA) reader. The color intensity is proportional to the content of the analyte in the sample, and the concentration of the analyte in the sample is calculated by plotting a standard curve.

Application

This kit is used to detect the concentration of human natural killer (NK) antigen in serum, plasma and other samples for research purposes only.

Testing Scope

0.312-20ng/mL

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Product Number: ELK054

Shipping and Storage

1. Reagent kit storage: 2-8℃.
2. Validity period: 6 months.

Component

Description

This kit uses the double antibody sandwich method to determine the expression of human Newcastle disease virus (NDV) in specimens. Using purified Newcastle disease virus (NDV) antibodies coated on microplates, solid-phase antibodies can be prepared that can bind to Newcastle disease virus (NDV) in the sample. After washing to remove unbound antigens and other components, they can then bind to HRP labeled antibodies to form antibody antigen enzyme labeled antibody complexes. After thorough washing, TMB substrate is added for color development. TMB is converted to blue under the catalysis of HRP enzyme and to the final yellow under the action of acid. Measure the absorbance (OD value) at a wavelength of 450nm using an enzyme-linked immunosorbent assay (ELISA) reader, and compare it with the CUTOFF value to determine the presence or absence of human Newcastle disease virus (NDV) in the specimen.

Application

This kit is used to determine the expression of Newcastle disease virus (NDV) in human serum, plasma, cells, tissues, and related liquid samples.

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