Product Number: S080183

Shipping and Storage

Wet ice transportation; Stored at 2-8 ℃, with a validity period of 12 months.

Component

Description

Protein A is a surface protein found on the cell wall of Staphylococcus aureus with a molecular weight of 42 kDa; Protein G is an immunoglobulin-binding protein expressed by C or G-type streptococcal bacteria. Protein A and Protein G have similar functions and can specifically bind to mammalian immunoglobulins (Immunoglobulin, Ig). Recombinant-modified Protein A and Protein G can be combined with gel in a certain manner for use in immunoprecipitation or antibody purification. Protein A gel is suitable for binding human IgG1, IgG2, IgG4, mouse IgG2a, IgG2b, etc., while Protein G gel is suitable for binding human IgG1, IgG2, IgG3, IgG4, mouse IgG1, IgG2a, IgG2b, IgG3, rat IgG1, IgG2a, IgG2b, IgG2c, and other polyclonal antibodies (specific details are provided in Appendix 1).

The product utilizes a Protein G protein labeling gel developed and produced in-house. Compared to similar products on the market, this product exhibits higher antibody binding efficiency and lower non-specific binding rates. When paired with an optimized buffer solution, it enables convenient and efficient immunoprecipitation experiments. It can be widely applied for the separation and purification of target proteins in samples such as cell lysates, cell supernatants, serum, and ascites.

Application

Protein immunoprecipitation, protein co-immunoprecipitation, protein purification and separation.

Features

1. Product content: 50% (v/v) agarose gel is stored in special preservation solution.
2. Gel type: 6% cross-linked agarose gel.
3. Conjugated protein: Protein G.
4. Molecular weight of conjugated protein: ~25kDa(Protein G).
5. Binding protein ability: >1mg mouse antibody/ml gel.
6. Specificity: Antibodies from different species, including mice, humans, rats, goats, sheep, and cows.
7. Gel size: 30~150μm
8. Elution method: Acid washing or 1 x protein loading buffer (reduction type) elution. Note: If 1×protein loading buffer (reduced) is used for elution, the heavy chain (~50kDa) and light chain (~25kDa) of the antibody will be denatured and released from the gel.

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Product Number: BAB-250g

Product details

Formula of culture medium (per liter); pH7.3 ± 0.1(25℃)

Ancillary Reagents

Sterile Defidrinated Sheep Blood

Instructions

Weigh 33.0g of this product into 1L of distilled water or deionized water (the preparation amount can be increased or decreased proportionally), heat and boil until completely dissolved, package, sterilize at 121℃ for 15 minutes or 115℃ for 30 minutes, shake well after sterilization to prevent agar from settling at the bottom of the vessel and solidifying, cool to 50-55℃, and slowly add 5-7% sterile defatted sheep blood that has been insulated at 35℃ along the bottle wall for aseptic operation. Gently shake well, pour onto a plate, solidify, and set aside.

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Product Number: HR10

Shipping and Storage 

 Store at room temperature and dry.

Description

High Resolution Agarose has high resolution for PCR products and small DNA fragments, which can be comparable to polyacrylamide and is suitable for separating 20 bp -800 bp DNA fragments.

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Product Number: LM05, LM10, LM25, LM50, LM100

Shipping and Storage 

 Store at room temperature and dry.

Description

The modified low melting point agarose has a finer structure and higher resolution, which is suitable for DNA/RNA electrophoresis. Small fragments of DNA have high resolution for PCR products.

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LE Agarose
LE, Multi-purpose Agarose
Agarose, Low electroendosmosis

Specification:

• CAS NO: 9012-36-6
• Appearance: White to off-white powder
• EEO: < 0.13
• Gelling Point: 36°C±1.5°C (1.5% gel)
• Melting Point: 88°C±1.5°C (1.5% gel)
• Solubility: Clear colorless solution at 1gr in 100ml water
• Moisture: ≤10%
• Gel strength: ≥ 1200g/cm2
• Sulfate:≤0.15%
• Ash:≤0.5%
• DNase/RNase/Protease: Not Detectable

Features：

1. High purity and Multi-purpose
2. Greener agarose choice
3. Low background and high resolution
4. Optimized gel strength for ease of gel preparation
5. Ideal for gel electrophoresis , chromatographic separations, and other life science applications.

Order Information:

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Manual

Product Number: EZ100

Description

EzAgarose™ Tablets are made with High purity Low EEO (LE) Agarose.

Pre-weighted (05g/tablet) no weighing required. For Multi-purpose, ready and easy to use.

EzAgarose™ is a line of cleaner agarose products with significantly reduced environmental impact at improved cost efficiency while delivering the same reliable high-quality user experience.

Specification

Features

• Pre-weighted tablet(0.5 g/ tablet), no weighing required 
• Made with high purity Green LE Agarose, multi-purpose 
• Blister packaging allows for full portability and easy dispensing 
• <1 min. safe hands-on time 

Package

• 0.5 gram per Tablet,
• 10 tablets(5g) per blister
• 10 blisters(200 tablets) per box.

Bulk OEM ODM is available!

Tablets LE Agarose Multi purpose, Agarose Tablets low EEO (LE) are a multi-purpose agarose in tablet format delivered in a convenient blister pack. Low electroendosmosis Agarose powder 0.5 g * 200 Tablets.

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