Product Number: ELK04

Shipping and Storage

1. Reagent kit storage: 2-8℃.
2. Validity period: 6 months.

Component

Description

This kit uses a double antibody sandwich method to determine the level of equine infectious anemia virus (IAV) in specimens. Coat a microplate with purified horse infectious anemia virus (IAV) antibody to produce solid-phase antibodies. Add IAV to the micropores coated with monoclonal antibodies in sequence, and then bind with HRP labeled IAV antibody to form an antibody antigen enzyme labeled antibody complex. After thorough washing, add substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and to the final yellow under the action of acid. The depth of color is positively correlated with the presence of infectious anemia virus (IAV) in the sample. Measure the absorbance (OD value) at a wavelength of 450nm using an enzyme-linked immunosorbent assay (ELISA) reader, and calculate the concentration of equine infectious anemia virus (IAV) in the sample using a standard curve.

Application

This kit is used to determine the content of infectious anemia virus (IAV) in horse serum, plasma, and related liquid samples.

Testing Scope

20pg/mL-480pg/mL

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Kit(Qualitative)

Product Number: ELK10746

Shipping and Storage

1. Reagent kit storage: 2-8℃, stored away from light and moisture.
2. Validity period: 6 months.

Component

Description

The reagent kit adopts a double antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). Add the specimen and HRP labeled detection antibody to the pre coated micropores with human immunodeficiency virus antigen, incubate and thoroughly wash. Using substrate TMB for color development, TMB is converted to blue under the catalysis of peroxidase and to the final yellow under the action of acid. Measure the absorbance (OD value) at 450nm wavelength using an enzyme-linked immunosorbent assay (ELISA) reader and compare it with the CUTOFF value to determine the presence or absence of human immunodeficiency virus (HIV) in the specimen.

Application

This kit is used to determine the expression of hepatitis B surface antigen (HBsAg) in human serum and plasma samples.

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Kit(Qualitative)

Product Number: ELK10556

Shipping and Storage

1. Reagent kit storage: 2-8℃.
2. Validity period: 6 months.

Component

Description

This kit uses the double antigen sandwich method to determine the expression of human hepatitis C virus antibodies (HCV Ab) in specimens. Using purified antigen coated microplates to prepare solid-phase antigens, they can bind to hepatitis C virus antibodies (HCV Ab) in the sample. After washing to remove unbound antibodies and other components, they can then bind to HRP labeled antigens to form antigen antibody enzyme labeled antigen complexes. After thorough washing, TMB substrate is added for color development. TMB is converted to blue under the catalysis of HRP enzyme and to the final yellow under the action of acid. Measure the absorbance (OD value) at 450nm wavelength using an enzyme-linked immunosorbent assay (ELISA) reader and compare it with the CUTOFF value to determine the presence or absence of human hepatitis C virus antibodies (HCV Ab) in the specimen.

Application

This kit is used to determine the expression of hepatitis C virus antibodies (HCV Ab) in human serum, plasma, and related liquid samples.

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Product Number: ELK10545

Shipping and Storage

1. Reagent kit storage: 2-8℃.
2. Validity period: 6 months.

Component

Description

This kit uses the double antibody sandwich method to determine the expression of human hepatitis B surface antigen (HBsAg) in samples. The purified antibody is coated on the microplate to make a solid phase antibody, which can combine with hepatitis B surface antigen (HBsAg) in the sample. After washing, the unconjugated antigen and other components are removed, and then combined with the HRP labeled antibody to form an antibody antigen enzyme labeled antibody complex. After thorough washing, the substrate TMB is added for color development. TMB is converted to blue under the catalysis of HRP enzyme and to the final yellow under the action of acid. Use the microplate reader to measure the absorbance (OD value) at the wavelength of 450nm and compare it with the CUTOFF value to determine whether there is human hepatitis B surface antigen (HBsAg) in the sample.

Application

This kit is used to determine the expression of hepatitis B surface antigen (HBsAg) in human serum and plasma samples.

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Product Number: ELK015001

Shipping and Storage

1. 2-8℃, stored away from light and moisture
2. Validity period: 6 months

Component

Description

The reagent kit adopts indirect enzyme-linked immunosorbent assay (ELISA). Add the specimen and HRP labeled detection antibody to the pre coated micro well with Treponema pallidum antigen, incubate and thoroughly wash. Using substrate TMB for color development, TMB is converted to blue under the catalysis of peroxidase and to the final yellow under the action of acid. Measure the absorbance (OD value) at 450nm wavelength using an enzyme-linked immunosorbent assay (ELISA) reader and compare it with the CUTOFF value to determine the presence or absence of Treponema pallidum antibody (TP Ab) in the specimen.

Specimen collection and storage

1. Serum: Use test tubes without pyrogen and endotoxin, avoid any cell irritation during the operation, collect blood, centrifuge at 3000 rpm for 10 minutes, and quickly and carefully separate serum and red blood cells.
2. Plasma: anticoagulant with EDTA, citrate or heparin. Centrifuge at 3000 rpm for 30 minutes and collect the supernatant.
3. Cell supernatant: Centrifuge at 3000 rpm for 10 minutes to remove particles and polymers.
4. Tissue homogenate: Crush the tissue by adding an appropriate amount of physiological saline. Centrifuge at 3000 rpm for 10 minutes and collect the supernatant.
5. Storage: If the sample is not tested in a timely manner after collection, please pack it according to a single dose and freeze it at -20℃ to avoid repeated freezing and thawing. Thaw it at room temperature and ensure that the sample is evenly filled and thawed.

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Product Number: ELK03

Shipping and Storage

1. Reagent kit storage: 2-8℃.
2. Validity period: 6 months.

Component

Description

This test kit uses the double antibody sandwich method to determine the level of bovine circular Taylor disease (T. annua) in specimens. Coat a microplate with purified bovine T. annulata antibody to prepare a solid-phase antibody. Add T. annulata to the micropores coated with the antibody in sequence, and then bind with HRP labeled T. annulata antibody to form an antibody antigen enzyme-linked antibody complex. After thorough washing, add substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and to the final yellow under the action of acid. The depth of color is positively correlated with the presence of T. annulata in the sample. Measure the absorbance (OD value) at a wavelength of 450nm using an enzyme-linked immunosorbent assay (ELISA) reader, and calculate the concentration of T. annua in the sample using a standard curve.

Application

This kit is used to determine the content of T. annua in bovine serum, plasma, and related liquid samples.

Testing Scope

2pg/mL-90pg/mL

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Product Number: ELK02

Shipping and Storage

1. Reagent kit storage: 2-8℃.
2. Validity period: 6 months.

Component

Description

This kit employs the double-antigen sandwich method to determine the level of bovine circovirus antibody (Circovirus Ab) in samples. Purified antigens are coated onto microplates to create solid-phase antigens. The coated wells are sequentially added with circovirus antibody (Circovirus Ab), followed by binding with HRP-labeled antigens to form an antigen-antibody-enzyme-labeled antigen complex. After thorough washing, the substrate TMB is added for color development. TMB is converted into a blue color under the catalysis of HRP and further transformed into a final yellow color under acidic conditions. The intensity of the color is directly proportional to the level of circovirus antibody (Circovirus Ab) in the sample. The absorbance (OD value) is measured at a wavelength of 450nm using a microplate reader, and the concentration of bovine circovirus antibody (Circovirus Ab) in the sample is calculated via a standard curve.

Application

This kit is designed to measure the content of circovirus antibodies (Circovirus Ab) in bovine serum, plasma, and related liquid samples.

Testing Scope

4ng/L -220ng/L

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Product Number: ELK014670

Shipping and Storage

1. Storage: 2-8℃.
2. Validity period: 6 months.

Component

Note: Standard (S0 → S5) concentration was followed by:0,15,30,60,120,240 pg/mL.

Application

This HDC ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of HDC in the sample, this HDC ELISA kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus HDC concentration. The concentration of HDC in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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Shipping and Storage

1. Reagent kit storage: 2-8℃.
2. Validity period: 6 months.

Component

Description

This kit uses the double antigen sandwich method to determine the expression of Brucellosis specific antibody IgM (Brucellosis IgM) in specimens. Using purified antigen coated microplates to prepare solid-phase antigens, they can bind to Brucellosis specific antibody IgM (Brucellosis IgM) in the sample. After washing to remove unbound antibodies and other components, they can then bind to HRP labeled antigens to form antigen antibody antigen complexes. After thorough washing, TMB substrate is added for color development. TMB is converted to blue under the catalysis of HRP enzyme and to the final yellow under the action of acid. Measure the absorbance (OD value) at a wavelength of 450nm using an enzyme-linked immunosorbent assay (ELISA) reader, and compare it with the CUTOFF value to determine the presence or absence of Brucellosis specific antibody IgM in the specimen.

Application

This kit is used to determine the expression of Brucellosis specific antibody IgM (Brucellosis IgM) in mouse serum, plasma, and related liquid samples.

Testing Scope

3ng/L -150ng/L

Specimen requirements

1. Extract the specimen as soon as possible after collection, according to relevant literature, and conduct experiments as soon as possible after extraction. If the experiment cannot be conducted immediately, the specimen can be stored at -20 ℃, but repeated freezing and thawing should be avoided.
2. Samples containing NaN3 cannot be detected because NaN3 inhibits the activity of horseradish peroxidase (HRP).

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Product Number: ELK01

Shipping and Storage

1. Reagent kit storage: 2-8℃.
2. Validity period: 6 months.

Component

Description

This kit employs the double-antibody sandwich method to determine the level of Rift Valley fever virus (RVFV) in samples. Purified anti-RVFV antibodies are coated onto microplates to create solid-phase antibodies. The wells coated with monoclonal antibodies are sequentially added with RVFV, followed by binding with HRP-labeled anti-RVFV antibodies to form an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, a substrate TMB is added for color development. TMB is converted to blue under the catalysis of HRP and further transformed into the final yellow color under acidic conditions. The intensity of the color is positively correlated with the RVFV level in the sample. The absorbance (OD value) is measured at a wavelength of 450nm using a microplate reader, and the RVFV concentration in the sample is calculated via the standard curve.

Application

This kit is designed for the determination of Rift Valley fever virus (RVFV) content in human serum, plasma, and related liquid samples.

Testing Scope

2pg/mL-80pg/mL

Specimen requirements

1. Extract the specimen as soon as possible after collection, according to relevant literature, and conduct experiments as soon as possible after extraction. If the experiment cannot be conducted immediately, the specimen can be stored at -20℃, but repeated freezing and thawing should be avoided.
2. Samples containing NaN3 cannot be detected because NaN3 inhibits the activity of horseradish peroxidase (HRP).

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