Product Number: CLE2000

Shipping and Storage

1. Transportation: By air or Refrigerated sea transportation.
2. Storage: Cold storage (2℃~ 8℃), avoid exposure to light.
3.  Shelf life: 2 year with cold storage.
4. Package: 25kg /drum.

Description

Coagulase is derived from the Kluyveromyces lactis strain. It’s a food grade enzyme which produced from submerged fermentation. The clotting of milk by coagulase can be divided into two stages. First, the enzyme specific hydrolysis the peptide bond in Phe105-Met106 from k-casein polypeptide chain in milk to form stable para-k casein. Second, para-k casein, α-casein and β-casein will precipitate to form clotting with the action of Ca+2.

Application

 This product is mainly used in cheese processing, which can make milk clotting.

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Product Number: RA02

Description

Our Ribonuclease A Solution is a non-specific ribonuclease derived from bovine pancreas that has been modified through protein engineering technology. It is obtained through yeast expression and purification. It does not contain bacterial endotoxins expressed by prokaryotes. Functionally, RNase A is a ribonuclease that can hydrolyze the phosphodiester bond between the 5 '- ribose on a nucleoside and the phosphate group on the adjacent pyrimidine nucleoside 3' - ribose. The resulting 2 ', 3' - cyclic phosphate can be hydrolyzed into the corresponding 3 '- nucleoside phosphate. Therefore, single stranded RNA can be specifically degraded at the positions of C and U nucleotide residues. RNase A is very stable. RNase A exhibits high efficacy when acting on single stranded RNA.

This product is widely used to remove RNA contamination from DNA or protein samples.

This product is free from contamination by endonucleases and exonucleases, as well as protease contamination.

Quality Index

Note

For your safety and health, please wear lab clothes and protective gloves when using.

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Proteinase K solution

Product Number: PK02 Solution Molecular bio grade

Shipping and Storage

Proteinase K solution suggest ship with blue ice.

After opening the package, if keep at 2-8°C for more than one week, it is recommended to sterilize by filtration. PES or PVDF membranes with low protein binding are recommended in sterile filtration.

Description

Proteinase K is a broad-spectrum serine protease originally isolated from fungus Engyodontium album. Proteinase K is commonly used in molecular biological and biopharmaceutical applications to remove protein contamination from preparations of highly native undamaged nucleic acid because it rapidly and effectively inactive nuclease that might degrade the DNA or RNA even in the presence of denaturing reagents. MEBEP recombinant proteinase K is a mutant to the native protease that result in improved specific activity, higher yield and wider range of pH/temperature with optimal activity. The large scale recombinant preparation has advantages in lot-to-lot consistency, superior purity and cost-efficiency. DNA-free nature of recombinant Proteinase K made it well-suited in isolating DNA and RNA templates. Recombinant proteinase K is widely used for general digestion of proteins and Chemo-enzymatic peptide synthesis in molecular biology, molecular diagnostic and biopharmaceutical applications. Proteinase K is one of important enzymes in various industries like leather, cosmetics, food and feed etc.

Quality Control

• 1.DNase Activity: none detectable enzyme activity with λ DNA after 6 hours incubation at 37°C.
• 2.RNase Activity: none detectable of ribonuclease activity after 16 hours incubation at 25°C.
• 3.Protein Purity: > 95% (Native-PAGE and SDS-PAGE assay).

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Product Number: YF01

Shipping and Storage

1. Transportation: The reagent kit must be transported under frozen conditions.
2. Storage: Store at -20℃ and avoid repeated freezing and thawing. The freeze-thaw cycle of the reagent kit shall not exceed 7 times.
3. Validity period: 12 months, please use within the validity period.

Component

Description

This reagent uses real-time fluorescence PCR technology and is suitable for nucleic acid detection of yellow fever virus (YFV) extracted from clinical samples, environmental samples, and virus isolates. Each reaction system contains specific primers and fluorescent probes for detecting the yellow fever virus (YFV) gene. By collecting the fluorescent signal generated by PCR amplification, qualitative detection of YFV nucleic acid can be quickly completed.

In addition, specific primers and fluorescent probes for internal standard control of human clinical specimens were added to each reaction system to monitor the collection, transportation, and extraction process of the test samples, indicating false negatives in the detection results.

Application

This kit is suitable for qualitative detection of yellow fever virus nucleic acid extracted from clinical samples, environmental samples, and virus isolates. The experimental results only provide reference for basic research and are not used as clinical diagnostic basis.

Yellow fever is an acute infectious disease caused by the yellow fever virus, mainly transmitted through mosquito bites. The disease has a high mortality rate and strong infectivity, and has been included as one of the quarantine infectious diseases regulated by the World Health Organization.

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Product Number: RVF01

Shipping and Storage

1. Transportation: The reagent kit must be transported under frozen conditions.
2. Storage: Store at -20℃ and avoid repeated freezing and thawing. The freeze-thaw cycle of the reagent kit shall not exceed 7 times.
3. Validity period: 12 months, please use within the validity period.

Component

Description

This reagent uses real-time fluorescence PCR technology and is suitable for detecting Rift Valley fever virus nucleic acid extracted from whole blood, serum, and their cultures. Each reaction system contains specific primers and fluorescent probes for detecting the genes of Rift Valley fever virus. By collecting the fluorescent signals generated by PCR amplification, qualitative detection of Rift Valley fever virus nucleic acid can be performed.

In addition, specific primers and fluorescent probes for internal standard control of human clinical specimens were added to each reaction system to monitor the collection, transportation, and extraction process of the test samples, indicating false negatives in the detection results.

Application

This kit is suitable for qualitative detection of Rift Valley fever virus nucleic acid extracted from whole blood or serum and their cultures. The experimental results only provide reference for basic research and are not used as clinical diagnostic basis.

Rift Valley fever is an acute febrile animal disease caused by the Rift Valley fever virus. The virus is mainly transmitted by mosquitoes and can infect humans and animals. After infection, people usually experience symptoms such as fever, headache, bleeding, and shock.

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Benzo Nuclease is a kind of no-specific endonuclease. The Benzo Nuclease genetically engineered endonuclease from Serratia marcescens.

Benzo Nuclease degrades all kinds of DNA and RNA (double stranded, single stranded, linear and circular and supercoil) but without proteolytic activity. It is effective over a wide range of conditions and possesses an exceptionally high specific activity.

Benzo Nuclease completely digests nucleic acids to 5'monophosphate terminated oligonucleotides 2 to 5 bases in length (below the hybridization limit), which is ideal for removal of nucleic acids from recombinant proteins, enabling compliance with FDA guidelines for nucleic acid contamination. The ability of Benzo Nuclease to rapidly hydrolyze nucleic acids makes the enzyme an excellent choice for viscosity reduction to reduce processing time and increase yields of protein. For example, the enzyme is compatible with BugBuster® and PopCulture® Protein Extraction Reagents and can therefore be added along with these reagents to eliminate viscosity and remove nucleic acids from E. coli extracts. The enzyme consists of two subunits of 30 kDa each. It is functional between pH 6 and 10 and from 0-42°C and requires 1-2mM Mg2+ for activation. The enzyme is also active in the presence of ionic and non-ionic detergents, reducing agents, PMSF (1 mM), EDTA (1 mM) and urea (relative activity depends on specific conditions). Activity is inhibited by>150 mM monovalent cations,>100 mM phosphate,>100 mM ammonium sulfate, or>100 mM guanidine HCl.

• Source: Serratia marcescens
• Molecular weight: 27. 9 KD (SDS)
• Purity: ≥90%, (Ultra≥99%)(SDS-PAGE)
• PI: 6. 85
• Optimum pH: 8. 0
• Optimum temperature: 37°C
• Cofactor: 1～10mM Mg2+
• Form: Liquid enzyme is the clear Solution(Contain Glycerin)
• Solid enzyme is the white lyophilized powder.
• Concentration: 1000 U/µl.
• Activity: ≥1000U/μl
• Specific activity: ≥1. 0x106U/mg protein
• Protease: Not detectable
• Microbial limit: aerobe<10CFU/100 KU Yeast and mold: <10CFU/100 KU
• Endotoxin test: (Gel Clot LAL Assay)<0. 25EU/1000U

Storage Buffer

20 mM Tris-Cl(pH 8. 0), 2 mM MgCl2, 2 mM NaCl, 50%Glycerin

Dilution Buffer

20 mM Tris-Cl(pH 8. 0), 2 mM MgCl2, 2 mM NaCl.

Unit definition

One unit is defined as the amount of enzyme that causes a ΔA260 of 1. 0 in 30 minutes, which corresponds to complete digestion of 37 µg DNA.

Application

• Its high intrinsic activity and broad substrate tolerance make the endonuclease an ideal tool in a variety of biotechnological and pharmaceutical applications:
• removal of nucleic acid from protein samples
• Elimination of nucleic acids from recombinant proteins
• Purification of protein fragments from inclusion bodies;
• Sample preparation in western blotting or two-dimensional gel electrophoresis
• Viscosity reduction in protein extracts.

Shipping and Handling

Liquid enzyme: Ship with Blue Ice, Storage at -20°C
Lyophilized powder: Ship at room temperature, storage blow 4°C

Guarantee

2 years

CAS: 9025-65-4

EC: 3.1.30.2

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Description

Pancreatic Ribonuclease (RNase A or RNase 1) catalyzes the cleavage of the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide forming a 2',3'-cyclic phosphate which may then be hydrolyzed to the corresponding 3'-nucleoside phosphate. Ribonuclease A has a molecular weight of 13,700 daltons. It operates in an optimum pH range of 7.0-7.5.

The ultrapure Pancreatic Ribonuclease has been further purified by the affinity chromatography method to remove trace contaminating proteases.

Specifications

High-quality RNase A

High purity RNAse A offers OEM and bulk packages.

High-quality Ribonuclease A

DNase and RNase manufacturers offer OEM and bulk!

Source: Bovine Pancreas

EC: 3.1.27.5

Form: lyophilized powder

Activity: ≥ 50 Kunitz units/mg powder

Storage

Store in an airtight container, at a temperature of 2°C to 8°C.

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Product Number: PK01                           Lyophilized powder     Molecular bio grade

Shipping and Storage

Shipping under room temperature and Store at 4 to -20°C recommended.

Description

Proteinase K is a broad-spectrum serine protease originally isolated from fungus Engyodontium album. Proteinase K is commonly used in molecular biological and biopharmaceutical applications to remove protein contamination from preparations of highly native undamaged nucleic acid because it rapidly and effectively inactive nuclease that might degrade the DNA or RNA even in the presence of denaturing reagents. MEBEP recombinant proteinase K is a mutant to the native protease that result in improved specific activity, higher yield and wider range of pH/temperature with optimal activity. The large scale recombinant preparation has advantages in lot-to-lot consistency, superior purity and cost-efficiency. DNA-free nature of recombinant Proteinase K made it well-suited in isolating DNA and RNA templates. Recombinant proteinase K is widely used for general digestion of proteins and Chemo-enzymatic peptide synthesis in molecular biology, molecular diagnostic and biopharmaceutical applications. Proteinase K is one of important enzymes in various industries like leather, cosmetics, food and feed etc.

Preparation

1. The Molecular Biology Grade lyophilized powder is NOT sterile.
2. Stock solution can be prepared as 40-80 mg/ml in dilution buffer [20mM Tris-HCl (pH 7.4) , 1 mM CaCl₂] or [20mM Tris-HCl (pH 7.4) , 1 mM CaCl₂, 2% Glycerol], sterilized using a 0.22 μm filter and supplied at final concentration of 20-40mg/ml in 50% sterilized Glycerol. Store in aliquots at wide temperature range from 24°C to -80°C.
3. PES or PVDF membranes with low protein binding are recommended in sterile filtration.

Quality Control

1. DNase Activity: none detectable enzyme activity with λ DNA after 6 hours incubation at 37°C.
2. RNase Activity: none detectable of ribonuclease activity after 16 hours incubation at 25°C.
3. Protein Purity: > 95% (Native-PAGE and SDS-PAGE assay).

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The Hot Start Taq DNA Polymerase is a recombinant Taq DNA polymerase that has been chemically modified by the addition of heat-labile blocking groups to its amino acid residues. The enzyme is inactive at room temperature, avoiding the extension of non-specifically annealed primers or primer dimers and providing higher specificity of DNA amplification. The functional activity of the enzyme is activated in 10-minute incubation at 95°C. The activated enzyme maintains the same functionality as Taq DNA polymerase: Has the 5′to 3′DNA polymerase activity and 5′to 3′exonuclease activity without 3′-5′exonuclease activity. The extending speed is 1 kb/30 sec. There is an "A" on the 3′end. The PCR product can be cloned in TA vector.

Applications

Hot start PCR amplification

Specific amplification of complex cDNA and genomic template

The amplification from low copy number DNA template

Real-Time PCR

Multiplex PCR

Generation of PCR products for TA cloning

Quality Control

Functional absence of double and single-stranded endonuclease activity;

Purity>99% test by SDS gel electrophoresis;

Each lot of Hot Start Taq DNA Polymerase is assayed for amplification from as little as 10 ng of human genomic DNA; Retain full activity at room temperature for one week; No host DNA residue.

Concentration: 5 u/μl

Storage: Store at -20°C

Reaction Mixture Set Up

Package: This Hot Start Taq is always supplied by Bulk package, if you want the small package it is available.

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