Product Number: EK0227

Shipping and Storage

The adsorption column DF is stored at 2-8℃, while the remaining components are stored at room temperature (15-30℃).

Component

Description

The basic principle of this kit is that after treatment with bisμLfite, DNA can be converted from unmethylated cytosine to uracil, while methylated cytosine remains unchanged.

This reagent kit adopts an alkaline denaturation treatment method, which can denature DNA double stranded under mild conditions, ensuring DNA integrity while improving conversion efficiency. The conversion efficiency can reach over 99.5%. At the same time, by combining the conversion product with the adsorption column, purified DNA is recovered from the bisμLfite modified solution. The recovered DNA has high purity and good integrity, and can be directly used for downstream experiments such as sequencing, methylation PCR detection, and chip analysis.

Features

1. High conversion efficiency: The conversion efficiency of unmethylated cytosine is greater than 99.5%
2. High nucleic acid integrity: minimal damage to nucleic acid, beneficial for downstream testing
3. High recovery rate: recovery rate greater than 80%
4. High sample compatibility: compatible with DNA and cfDNA with an input range of 100pg-2ug.

Materials required but not supplied

Anhydrous ethanol, PCR tube, EP tube, centrifuge.

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Product Number: YTK001

Shipping and Storage

Stored at -20℃, valid for at least one year. Except for Carrier DNA, it is stored at 4℃ and valid for 3 months. DMSO can also be stored at room temperature.

Component

Description

Our company's Competitive Cell Preparation and Transformation Kit for Saccharomyces Cerevisiae is a rapid, convenient, and efficient kit for preparing highly active brewing yeast competent cells and performing plasmid transformation. This kit provides almost all the reagents required for the preparation and transformation of competent cells, except for the culture medium, without the need for dilution and preparation. The operation is simple and fast, and the preparation of competent cells can be completed within 30 minutes after yeast cultivation. It can be used for yeast hybridization experiments and yeast library construction experiments. When transforming brewing yeast, use the buffer solution provided in the reagent kit to culture yeast cells in the state to be transformed. Then mix the competent cells with the plasmid DNA and carrier DNA to be transformed, and incubate them with the transformation solution for transformation. Carrier DNA is a short linear single stranded DNA that promotes the entry of plasmids into yeast cells during the uptake of exogenous plasmid DNA, and also protects plasmids from degradation by DNA enzymes.

Saccharomyces cerevisiae is a single celled eukaryotic microorganism belonging to the genus Saccharomyces and the species Saccharomyces cerevisiae. Saccharomyces cerevisiae is a widely studied and used eukaryotic model with a genome of approximately 1.2 × 10⁷bp. The nucleus contains 16 chromosomes and approximately 6000 ORFs, with only 4% of yeast genes having introns and a simple genetic background. Brewing yeast has growth characteristics similar to prokaryotes, making it easy to cultivate and perform genetic operations. It is a model eukaryotic organism, known as the "Escherichia coli" of eukaryotes. Brewing yeast can exist in a haploid state, making it easier to perform genotype phenotype analysis and efficient homologous recombination, making it easy to edit genome sequences for high-throughput genetic analysis. The expression system of brewing yeast has a certain degree of post-translational processing ability when expressing exogenous genes. The harvested exogenous proteins undergo folding processing and glycosylation modification to a certain extent, which is beneficial for maintaining protein activity and stability. Moreover, exogenous genes can be secreted and expressed in brewing yeast, and the secretion of expressed products outside the cell is not only beneficial for purification, but also avoids the accumulation of large amounts of products inside the cell.

During the transformation of brewing yeast, appropriate plasmids can be selected based on the nutritional deficiency type mutations of the strain. Generally speaking, if specific components of the culture medium (amino acids, purines, or pyrimidines) are lacking, mutant strains cannot grow. Using plasmids complementary to the mutant gene of the strain can enable the transformant to grow on the culture medium lacking specific components. Usually, transforming 1µg plasmid can produce>103 transformants, and the transformation efficiency may vary among different strains of brewing yeast.

Please refer to Figure 1 for the transformation effect of using our company's brewing yeast competent cell preparation and transformation kit.

The transformation effect diagram of Yeast Transformation Kit (Saccharomyces cerevisiae).

A. After 48 hours of cultivation in pGAL1,10-α factor-MCS-His-MCS-Flag URA vector using yeast INVSc1 competent cells prepared using Yeast Transformation Kit (Saccharomyces cerevisiae), plates were prepared. B. The colony in Figure A was subjected to electrophoresis using D7279 yeast colony PCR kit (enzymatic hydrolysis) after colony PCR. Mix 1μg of plasmid DNA and 10μL of heat denatured Carrier DNA, and add them to 100μL of competent cells prepared using this kit. Gently tap the bottom of the tube with your fingers and mix well; Add 600µL Buffer C and incubate in a 30℃ water bath for 30 minutes. After incubation, add 70µL DMSO and gently mix up and down. Incubate in a 42℃ water bath for 15 minutes, then immediately incubate in an ice bath for 3-5 minutes. Centrifuge at room temperature of approximately 12000-14000g for 10 seconds, discard the supernatant, gently add 100µL Buffer A and resuspend the bacterial cells. Finally, coat all onto nutrient deficient medium plates without Uracil and invert at 30℃ for 2-3 days until a single colony appears. Subsequently, PCR validation was performed using the D7279 yeast colony PCR kit (enzymatic hydrolysis). 561bp is the amplification band of the target gene fragment. M, DNA Ladder. 1-10, experimental group (PCR template is yeast single colony lysate),+, positive control; -Negative control. The actual usage effect of this product may vary due to differences in experimental conditions, materials, etc. The effects shown in the picture are for reference only.

This kit can be used multiple times, and small and medium packages can be prepared and used for competent cells that can undergo 50 and 250 transformations, respectively.

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Product Number: ARG-100T

Shipping and Storage

Stored at -20ºC, valid for one year. The Amplex Red and enzyme mixture must be stored away from light.

Component

Description

The Amplex Red Glycerol Assay Kit developed by our company is a probe based kit that uses fluorescence or absorbance detection to quickly and sensitively detect the glycerol content in serum, plasma, tissue or cell samples, urine, biological fluids, tissue or cell culture supernatants, and other samples. This kit only detects the content of free glycerol and does not detect the glycerol content in triglycerides.

Glycerol, also known as glycerol or glycerol, is a colorless, odorless, sweet, clear, viscous liquid. The molecular formula of glycerol is C₃H₈O₃, with a molecular weight of 92.09. The chemical structure of glycerol is different from that of carbohydrates and does not belong to the same category of substances. Each gram of glycerol can generate 4 calories when completely oxidized, and glycerol is usually absorbed by the human body without changing blood sugar and insulin levels. Glycerol is a commonly used sweetener and moisturizer in the food processing industry, mostly found in sports food and dairy substitutes. Due to its ability to increase the moisture content in human tissues, glycerol can enhance the body's ability to move in high heat environments. Glycerol is widely used in the production of food, beverages, solvents, drugs, and cosmetics, therefore its quantification is of great significance in research and development.

Glycerol is the main component of triglycerides, the most important storage method for fats, and an important metabolite produced during the oxidation and synthesis processes of energy metabolism. When the human body ingests fat, most of it is emulsified into small particles by bile. Lipases secreted by the pancreas and small intestine hydrolyze the fatty acids in the fat into free fatty acids and monoglycerides. After absorption, monoglycerides and long-chain fatty acids are re synthesized into triglycerides in small intestinal cells. Under physiological conditions, triglycerides are hydrolyzed by lipolysis to produce glycerol and free fatty acids (FFA), which are then released into the bloodstream. The generated glycerol cannot be reabsorbed and utilized by adipose tissue like fatty acids, so the levels of glycerol and free fatty acids in the blood are important indicators for measuring lipolysis levels and are also important indicators in the development of many related drugs.

The Amplex Red in this kit is a highly sensitive fluorescent probe for H2O2. In the presence of horseradish peroxidase (HRP), Amplex Red can react 1:1 with H2O2 to produce a strong red fluorescent substance called Resorufin. The maximum excitation wavelength of the test brine is 571nm, the maximum emission wavelength is 585nm, and there is strong visible light absorption at the excitation wavelength. Therefore, this kit can be tested using both fluorescence and absorbance methods.

The detection principle of this reagent kit is shown in Figure 1. In the presence of ATP, glycerol is phosphorylated by glycerol kinase (GK) to glycerol-3-phosphate. The generated glycerol-3-phosphate then undergoes an oxidation reaction with oxygen gas under the action of glycerol phosphate oxidase (GPO) to produce dihydroxyacetone phosphate (DHAP) and H₂O₂. The content of glycerol is finally detected by detecting the fluorescence intensity or absorbance of the reaction product of H₂O₂ and Amplex Red, chlorpromazine. The fluorescence intensity and absorbance of test brine are directly proportional to the content of glycerol in the sample.

Figure 1. Schematic diagram of glycerol assay kit (ARG-100T) for detecting glycerol

This kit provides a standard solution of glycerol, which can be used to calculate the glycerol content in the sample by setting a standard curve. The detection effect of this reagent kit on glycerol standard is shown in Figure 2.

Figure 2. The standard curve for glycerol detection using the Glycerol Assay Kit (ARG-100T). The left image shows absorbance detection, and the right image shows fluorescence detection. The detection data in the figure shows a 60 minute reaction at 37ºC in the dark, and the value may be slightly lower after 30 minutes of reaction. The measured data may vary due to differences in experimental conditions, testing instruments, etc. The data in the figure is for reference only.

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Product Number: QDHPV16/18

Shipping and Storage

1. Store below 30°C. It is valid for 12 months.
2. Date of manufacture and term of validity: see the label.
3. Transport at normal temperature, not suggested over 14 days.
4. Opened but not completely used HPV 16/18 PCR Master Mix should be stored at (-20±5) °C. It is recommended to separate in PCR tubes before refrigeration to avoid repeated freezing and thawing of all reagents next time. Storage time should not exceed 21 days.

Components

Note：Do not mix reagents from different batches.

Description

This kit uses real-time fluorescence PCR detection technology to achieve qualitative detection of HPV type 16 and type 18 in genitourinary tract secretion samples by using a pair of specific primers and a specific fluorescent probe in the conserved region shared by human papillomavirus (HPV) type 16 and type 18.

This kit sets internal control, to monitor the presence of PCR inhibitors in the samples by detecting whether the internal control is normal, so as to avoid PCR false negative result.

Application

This kit is used to qualitatively detect the nucleic acid of Human Papilloma Virus16/18 in male urethral swab samples, female cervical swab samples in vitro.

The test results can be used for auxiliary diagnosis of sexual transmission and reproductive tract infectious diseases caused by human papilloma virus type 16and type 18. The test results are only for clinical reference and should not be used as the sole criterion for clinical diagnosis.

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Product Number: QDHPV14

Shipping and Storage

1. Store below 30°C. It is valid for 12 months.
2. Transport at normal temperature, not suggested over 14 days. Opened but not completely used HPV PCR Master Mix should be stored at (-20±5) °C. It is recommended to separate in PCR tubes before refrigeration to avoid repeated freezing and thawing of all reagents next time. Storage time should not exceed 21 days.
3. Date of manufacture and term of validity: see the label.

Components

Note：1.Do not mix reagents from different batches.

2.The reaction system is lyophilized powder that contains all components required for fluorescence PCR, including Taq enzyme, primers, probes, dNTPs, and Mg²⁺.

3.Add 100μL Redissolved Diluent to the Positive Control, mix well, and use a handheld centrifuge to centrifuge briefly before use.

Description

This kit uses real-time fluorescence PCR detection technology to achieve qualitative detection of HPV genotypes 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68 in genitourinary tract secretion samples by using a pair of specific primers and a specific fluorescent probe in the conserved region shared by HPV genotypes 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68.The kit is provided with an Internal Control(IC), which can monitor whether there is PCR inhibitor in the sample to be tested by detecting whether the internal control is normal or not, so as to avoid false negative PCR.

The kit contains 4 bottles. The specific test items are as follows:

Application

This kit is suitable for qualitative detection of 14 high-risk HPV DNA (HPV16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68) in male urethral swab samples and female cervical swab samples in vitro.

Cervical cancer (CC) is the fourth most frequently diagnosed cancer and the fourth leading cause of cancer death in women worldwide. It has been demonstrated that persistent infection with various human papillomavirus (HPV)genotypes plays a major role in the development of high and low-grade cervical intraepithelial neoplasia (CIN) and CC. Human papilloma virus (HPV) is a small, double-stranded, circular DNA virus. HPV infection is considered the most common viral sexually transmitted infection worldwide. HPV has been classified genotypes 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68 as carcinogenic to humans. The test results can be used for auxiliary diagnosis of sexual transmission and reproductive tract infectious diseases caused by human papillomavirus genotypes 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68. The test results are only for clinical reference and should not be used as the sole criterion for clinical diagnosis.

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Product Number: PDK01

Shipping and Storage 

Please store the reagents at -20℃ and avoid repeated freeze-thaw cycles. The validity period is two years; Before use, it should be completely melted at room temperature and thoroughly inverted before centrifugation.

Components

Description

This kit utilizes a pair of specific primers for pig mitochondrial DNA, a specific fluorescent probe, and components such as Hot Start Taq DNA Polymerase and four types of monomeric nucleotides (dNTPs). PCR technology is used to amplify conserved genes in pig mitochondrial DNA, and external standard methods are used to detect mitochondrial DNA in the sample. The lower limit of DNA detection is 0.1pg/μl.

This reagent kit has no non-specific amplification for samples from cows, horses, sheep, chickens, ducks, rabbits, donkeys, mice, and geese.

Reagents and items that users need to bring themselves

1.1.5 ml centrifuge tube, 8-row or single tube PCR tube

2.Pipette and suction head (To avoid contamination between samples, please choose a pipette suction head containing a filter element)

3.Disposable gloves, protective equipment, and tissues

4.Desktop small centrifuge (can be equipped with rotors for centrifuging 1.5 ml centrifuge tubes and 2 ml centrifuge tubes)

5.Vortex oscillator

Note

1.Before use, completely dissolve the reagent at room temperature, mix it upside down and centrifuge briefly to allow the reagent to deposit to the bottom of the tube.

2.When preparing PCR reaction solution, please place the reagent on ice and avoid strong light exposure.

3.It is recommended to set at least three experimental areas from the preparation of reaction solution to the addition of detection samples, and conduct physical isolation.

3.1.Areas 1: Preparation and packaging of reaction solution.

3.2.Areas 2: Preparation of DNA for testing samples.

3.3.Areas 3: Add DNA from the test sample to the reaction solution for reaction and detection (PCR reaction tubes after amplification are strictly prohibited from being opened!)

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Product Number: MNK01

Shipping and Storage 

Freeze and store below -20℃ in a dark place, with a validity period of 12 months.

Components

Description

This reagent kit uses probe based quadruple real-time fluorescence quantitative PCR technology to design specific primers and fluorescent probes for nucleic acid sequences of sheep, duck, pig, and cattle derived components. Qualitative and quantitative detection of nucleic acid sequences of sheep, duck, pig, and cattle derived components in meat and meat products is determined through real-time fluorescence quantitative PCR (Taqman probe method) amplification curves.

Applicable instruments:

ABI series (ABI 7300/ABI 7500/Step One, etc.), Roche LightCycle series, Bole CFX 96, Beijing Kunpeng Gene X series, San Shi Biological Q162D, and other real-time fluorescence quantitative PCR instruments.

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Product Number: LAK01

Shipping and Storage 

Store at 4℃ in dark for 12 months

Description

Lysine is one of the essential amino acids in the human body, which can promote human development, enhance immune function, and improve the function of central nervous system tissues. Lysine is an essential alkaline amino acid. Due to the low content of lysine in cereal foods and their susceptibility to damage and deficiency during processing, it is called the first limiting amino acid. This reagent kit provides a simple detection method that can detect the content of lysine in various biological samples. The principle is that lysine in proteins has a free εNH2 reacts with ninhydrin reagent to generate a blue purple substance with a characteristic absorption peak at 570nm; Calculate the lysine content by measuring the absorbance at 570nm.

Components

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Product Number: QDHC01

Shipping and Storage 

1.Store below 30°C. It is valid for 12 months.

2.Transport at normal temperature, not suggested over 14 days.

3.Opened but not completely used HCMV PCR Master Mix should be stored at (-20±5)°C. It is recommended to separate in PCR tubes before refrigeration to avoid repeated freezing and thawing of all reagents next time. Storage time should not exceed 21 days.

Components

Note: 1.For the specific concentrations of HCMV quantitative standard substances 1~4, please refer to the given values in the kit.

2.Do not mix reagents from different batches.

3.Material Required but Not Provided:

3.1Applicable Instrument: ABI7500, ABI 7500 Fast and other Real-time fluorescence PCR instrument with FAM, VIC channels.

3.2Vortex shaker, palm centrifuge, pipette are needed.

Description

This kit utilizes real-time fluorescent PCR detection technology, and uses specific primers and probes for HCMV to achieve quantitative detection of HCMV DNA in serum or plasma samples. The kit is provided with an internal control(IC), which can monitor whether there is PCR inhibitor in the sample to be tested by detecting whether the internal control is normal or not, so as to avoid false negative PCR.

Sample Requirements

1.Applicable sample type: serum or plasma samples.

2.Sample collection:

2.1.Serum: Use a sterile syringe to draw 2mL of the subject's venous blood and inject it into a sterile centrifuge tube. Leave it at room temperature for no more than 4 hours. Centrifuge at 2000rpm for 5 minutes. Take the upper serum (be careful not to bring in red blood cells) and transfer it to another sterile centrifuge tube for use.

2.2.Plasma: 2mL of venous blood is drawn with a disposable sterile syringe and injected into a glass tube containing EDTA anticoagulant. Immediately invert the glass tube slightly and mix it for 5 to 10 times to fully mix the anticoagulant and venous blood. Centrifuge at 1500 rpm for 5 minutes; Suck the upper plasma and transfer it to a 1.5mL centrifuge tube for standby.

3.Sample storage and transportation：

The serum to be tested should be stored at 2~8°C for no more than 72 hours, and at -20°C for no more than 3 months. Samples are shipped in curling or ice-filled foam boxes.

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Product Number: RA100s

Shipping and Storage 

1.The complete reagent kit is stored in a dark place at 2-8℃. After initial use, it is recommended to store RNA HS Reagent at room temperature and avoid light; RNA HS Buffer storage at room temperature; RNA HS Standard 1 and 2 were stored at 2-8℃.

2.Adjust transportation methods according to different destinations.

Components

Description

RNA HS (High Sensitivity) Assay Kit is a simple, sensitive, and accurate RNA fluorescence quantitative detection kit. This reagent kit includes fluorescence detection reagents, buffer solutions, and RNA standards. This reagent kit has high selectivity for RNA, is not affected by dsDNA, and has excellent linear relationships with RNA samples in the 5-100 ng range. It can accurately quantify total RNA, rRNA, and mRNA samples with concentrations ranging from 250pg/µ l to 100ng/µ l, and has excellent tolerance to some conventional pollutants such as salt, free nucleotides, proteins, solvents, and detergents. This product is easy to operate and can be performed at room temperature. Before use, dilute the fluorescence detection reagent with buffer to a working solution, then add the RNA sample to be tested and detect it using a Qubit fluorescence meter.

Application

Total RNA、rRNA、mRNA from 250 pg/μl to 100ng/μl

Note

1.Fluorescent dyes have quenching issues, please try to avoid light as much as possible.

2.For detection reagents and RNA standards, mix them upside down before each use, and briefly centrifuge for 1-2 seconds to collect the reagents at the bottom of the tube.

3.To avoid degradation of RNA standards, please use RNA free consumables for the experiment and store the standards at 2-8℃ after the experiment is completed.

4.To ensure the accuracy of quantitative results, please use a calibrated pipette for operation.

5.Please perform quantitative testing at room temperature. Before use, place the components in the reagent kit at room temperature. During the experiment, please do not hold the PCR tube with your hands for a long time.

6.Please make sure to complete the testing of all samples within 3 hours of preparing the working solution to avoid deviation in the results caused by fluorescence quenching.

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