Product Number: PLK1201

Shipping and Storage

1. RNase A is stored in ready to use glycerol buffer solution, transported at room temperature, and upon receipt, stored at room temperature not exceeding 25℃ for at least 6 months, 4℃ for 12 months, and long-term storage at -20℃.
2. When used for the first time, all RNase A in the kit can be added to solution P1 (final concentration of 100μg/mL) and stored at 4℃ for about 3 months. If RNase A in solution P1 becomes inactive for a long time, there may be trace amounts of RNA residue in the extracted plasmid. Adding RNase A to solution P1 is sufficient.
3. SDS in solution P2 may precipitate when the ambient temperature is low, resulting in turbidity or precipitation. It can be heated in a 37℃ water bath for a few minutes to recover the clarification, and then remixed. Do not shake violently to avoid the formation of excessive foam.

Component

This reagent kit can be stored at room temperature for 12 months without affecting its effectiveness.

Description

This kit uses a unique high-yield SDS alkaline lysis formula to lyse cells, increasing plasmid yield by 1-2 times. The silicon matrix membrane inside the centrifugal adsorption column selectively binds to plasmid DNA in the solution under high salt and low pH conditions. Impurities and other bacterial components such as endotoxins are removed by passing protein solution and rinsing solution. Finally, the pure plasmid DNA is eluted from the silicon matrix membrane using low salt and high pH elution buffer. The extracted plasmid has high purity and has removed most endotoxins. In addition to being used for routine PCR, enzyme digestion, transformation and other experiments, it can also be directly used for general transfection experiments such as protoplast transfection. For cell lines with special high requirements for transfection, Adlai's endotoxin free plasmid medium/large extraction kit can be selected.

Feature

1. The specially improved high-yield buffer formula can increase plasmid yield by 1-2 times.
2. All the silicon matrix membranes in the centrifugal adsorption column are made of specially designed adsorption membranes, with minimal differences in adsorption capacity between columns and good repeatability. Overcoming the drawbacks of unstable membrane quality in domestic reagent kits.
3. The unique protein removal formula can efficiently remove residual nucleases, even strains with high nuclease content such as JM series and HB101 can be easily removed. Effectively prevent plasmids from being degraded by nucleases.
4. No toxic phenol, chloroform, or ethanol precipitation is required. Fast and convenient, 0.5-2 mg of pure high copy plasmid DNA can be quickly extracted from 150-300 ml of Escherichia coli LB (Luria Bertani) culture medium, with an extraction rate of 80-90%.

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Product Number: PLK0901

Shipping and Storage

Room temperature (10-30 ℃).

Component

Description

This kit is specifically designed for efficient and rapid extraction of plasmids from 15-50mL bacterial solution. On the basis of alkaline lysis of cells, a unique silicon matrix membrane adsorption technology is used to efficiently and specifically bind plasmid DNA. Each adsorption column can adsorb up to 250μg of plasmid DNA; At the same time, a special buffer system and endotoxin removal filter are used to effectively remove impurities such as endotoxins, genomic DNA, RNA, proteins, etc. The plasmid obtained from this kit has high purity and stable quality, and can be used for cell transfection as well as DNA sequencing, PCR， In vitro transcription, endonuclease digestion and other experiments.

Self provided reagents

Anhydrous ethanol, isopropanol.

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Product Number: PLK2001

Shipping and Storage

1. When using for the first time, add all RNaseA carried by the reagent kit to Buffer P1 (final concentration 100μg/ml) and store at 4℃. If RNaseA is inactivated in Buffer P1, the extracted plasmid may contain trace amounts of RNA residue. Adding RNase A to Buffer P1 is sufficient.
2. Buffer ER can be stored at 4℃ for one month. If it needs to be stored for a long time, it is recommended to store it at -20℃!
3. When the ambient temperature is low, SDS in Buffer P2 may precipitate and become turbid or precipitate. It can be heated in a 37℃ water bath for a few minutes to restore clarity. Do not shake violently to avoid excessive foam formation.
4. To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.

Components

Description

The conventional centrifugal column plasmid extraction kit is not suitable for the extraction of large plasmid DNA such as BAC/PAC/P1/Cosmid. This is mainly because the molecular weight of large plasmid DNA is very large, often exceeding 100kb, and generally has low copy number and low yield. The use of conventional centrifugal columns to adsorb membranes has low adsorption efficiency and yield, and passing the membrane will interrupt these large molecular weight plasmid DNA. This kit uses an improved alkaline lysis method to extract plasmid DNA from cultured bacteria. With a unique solution formula and Buffer ER, it only requires a few simple centrifugations to remove impurities such as proteins, polysaccharides, endotoxins, RNA, and obtain high-quality plasmid DNA. The OD260/280 of purified DNA is usually around 1.8, and the resulting plasmid can be directly applied in tasks that require high DNA purity, such as cell transfection and even animal in vivo experiments. The purification process in the later stage is operated in a 1.5ml centrifuge tube, which is simple, does not require special equipment, does not require column passing, and does not require phenol chloroform extraction; Plasmids released by bacterial lysis can be completely recovered without worrying about the loss of plasmid DNA. This method extracts and purifies plasmid DNA with minimal damage to plasmids. Even large plasmids or ultra large BAC/PAC plasmids with a size of 100kb or even 200kb can be effectively purified as long as they can be extracted by alkaline lysis. In addition, the solution type reagents can be scaled up or down in proportion for small/medium/large extraction. Finally, any small volume can be chosen to dissolve the plasmid, with a concentration of up to 3μg/μl.

Features

1. No need to use toxic reagents such as phenol and chloroform, and no need for ethanol precipitation. Rapid and convenient extraction of high-purity BAC plasmid DNA with a typical yield of 30-50μg can be achieved from 150ml of Escherichia coli LB (Luria Bertani) culture medium, with an extraction rate of 80-90%.
2. The obtained plasmids have high yield, high superhelix ratio, and good purity, and can be directly used for various molecular biology experiments such as transfection, sequencing, and library.
3. The endotoxin content is extremely low (<0.1EU/μg DNA) and can be directly applied to cell transfection

Application

Suitable for the preparation of large plasmids such as BAC/PAC/P1/Cosmid

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Product Number: PLK1901

Shipping and Storage

1. RNase A is stored in a ready to use glycerol buffer and transported at room temperature. Upon receipt, it should be stored at room temperature not exceeding 25℃ for at least 6 months, at 4℃ for 12 months, and for long-term storage at -20℃.
2. When using for the first time, add all RNase A carried by the reagent kit to Buffer YP1 (final concentration 100μg/ml) and store at 4℃. If RNase A is inactivated in Buffer YP1, the extracted plasmid may contain trace amounts of RNA residue. Adding RNase A to Buffer YP1 is sufficient.
3. When the ambient temperature is low, SDS in Buffer YP2 may precipitate and become turbid or precipitate. It can be heated in a 37℃ water bath for a few minutes to restore clarity. Do not shake violently to avoid excessive foam formation.
4. To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.

Components

Description

This reagent kit uses an improved SDS alkaline lysis method to lyse cells and combines Lyticase specific digestion of yeast cell walls. It can isolate high-purity plasmid DNA from yeast culture medium within 1 hour. After yeast collection, Lyticase is added to remove the cell wall, followed by alkaline lysis of the cells. The silica matrix membrane in the centrifuge adsorption column selectively binds to plasmid DNA in the solution under high salt and low pH conditions. Impurities and other bacterial components are then removed through Buffer PE and Buffer WB. Finally, the pure plasmid DNA is washed off the silica matrix membrane using low salt and high pH Buffer EB.

Features

1. The silicon matrix membranes inside the centrifugal adsorption column are all made of Adlai specially designed adsorption membranes, with minimal differences in adsorption capacity between columns and good repeatability. Overcoming the drawback of unstable membrane quality in domestic reagent kits.
2. Not fast, convenient, and does not require the use of toxic reagents such as phenol and chloroform, nor does it require ethanol precipitation. The obtained plasmids have high yield and good purity, and can be directly used for various molecular biology experiments such as enzyme digestion, transformation, PCR, in vitro transcription, sequencing, etc.

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Product Number: PLK1801

Shipping and Storage

1. When using for the first time, add all RNaseA carried by the reagent kit to Buffer P1 (final concentration 100μg/ml) and store at 2-8℃. If RNaseA is inactivated in Buffer P1, there may be trace RNA residues in the extracted plasmid. Adding RNaseA to Buffer P1 is sufficient.
2. When the ambient temperature is low, SDS in Buffer P2 may precipitate turbidity or sediment. It can be heated in a 37 ℃ water bath for a few minutes to restore clarity. Do not shake violently to avoid excessive foam formation.
3. To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.

Components

Description

This kit uses a unique high-yield SDS alkaline lysis formula to lyse cells, resulting in a 1-2 fold increase in plasmid production. The silicon matrix membrane in the centrifugal adsorption column selectively binds plasmid DNA in the solution under high salt and low pH conditions, and then removes impurities and other bacterial components through Buffer PE and Buffer WB. Finally, the pure plasmid DNA is eluted from the silicon matrix membrane using low salt and high pH Buffer EB.

Features

1. The specially improved high-yield buffer formula can increase plasmid production by 1-2 times.
2. The unique Buffer PE formula can efficiently remove residual nucleases, even strains with abundant nuclease content such as JM series and HB101 can be easily removed. Effectively preventing plasmid degradation by nucleases.
3. Fast and convenient, without the need for toxic reagents such as phenol and chloroform, and without the need for ethanol precipitation. The obtained plasmids have high yield and good purity, and can be directly used for various molecular biology experiments such as enzyme digestion, transformation, PCR, in vitro transcription, sequencing, etc.

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Product Number: PLK1401

Shipping and Storage

1. When using for the first time, add all RNase A carried by the reagent kit to Buffer P1 (final concentration 100μg/ml) and store at 4℃. If RNase A is inactivated in Buffer P1, the extracted plasmid may contain trace amounts of RNA residue. Adding RNase A to Buffer P1 is sufficient.
2. Buffer ER can be transported at room temperature and stored at 4℃ for one month. It can be stored for a long time at -20℃.
3. When the ambient temperature is low, SDS in Buffer P2 may precipitate and become turbid or precipitate. It can be heated in a 37℃ water bath for a few minutes to restore clarity. Do not shake violently to avoid excessive foam formation.
4. To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.

Components

Description

This reagent kit extracts plasmid DNA from cultured bacteria using alkaline lysis method. It uses a unique solution formula and endotoxin clearance reagent, and only requires a few simple centrifugations to remove impurities such as proteins, polysaccharides, endotoxins, RNA, etc., to obtain high-quality plasmid DNA. The OD260/280 of purified DNA is usually around 1.8, and the resulting plasmid can be directly applied in tasks that require high DNA purity, such as cell transfection and even animal in vivo experiments. The purification process in the later stage is operated in a 1.5ml small centrifuge tube, which is simple, does not require special equipment, does not require column passing, and does not require phenol chloroform extraction; Plasmids released by bacterial lysis can be completely recovered without worrying about the loss of plasmid DNA. This method extracts and purifies plasmid DNA with minimal damage to plasmids. Even large plasmids or ultra large BAC/PAC plasmids with a size of 10kb or even 100kb can be effectively purified as long as they can be extracted by alkaline lysis. You can choose to dissolve plasmids in any small volume, with a concentration of up to 5μg/μl. The super helix ratio can reach up to 95%, with no endotoxins and good transfection effect.

Features

1. No need to use toxic reagents such as phenol and chloroform, and no need for ethanol precipitation. Fast and convenient, 0.5-2mg of pure high copy plasmid DNA can be quickly extracted from 150-200ml of Escherichia coli LB (Luria Bertani) culture medium, with an extraction rate of 80-90%.
2. The obtained plasmid yield is high, with a super helix ratio of up to 95% and a concentration of up to 5μg/μl. Good purity, can be directly used for various molecular biology experiments such as enzyme digestion, transformation, PCR, in vitro transcription, sequencing, etc.
3. The endotoxin content is extremely low (<0.1EU/gDNA) and can be directly applied to cell transfection.

Application

Suitable for the preparation of large amounts of high-purity or transfection grade plasmids and the preparation of large BAC/PAC plasmids

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Product Number: PLK1301

Shipping and Storage

1. When used for the first time, add all RNase A carried by the kit to Buffer P1 (final concentration 100ug/ml) and store it at 4℃. If RNase A is inactivated in Buffer P1, the extracted plasmid may be mixed with trace RNA residues. Add RNase A to Buffer P1.
2. When the ambient temperature is low, SDS in Buffer P2 may precipitate and appear turbid or precipitated. It can be heated in a 37℃ water bath for a few minutes to restore clarity. Do not shake violently to avoid excessive foam formation.
3. Avoid volatilization, oxidation and pH change of reagents exposed to air for a long time, and close the cover of each solution in time after use.
4. Buffer ER is transported at room temperature. It can be stored at 4℃ for one month, and stored at -20℃ for a long time.

Components

Description

This kit uses an improved SDS alkaline lysis method to lyse cells. The crude extract is selectively combined with centrifugation to remove endotoxin through a unique buffer Er, and then the silicon matrix membrane in the centrifugal adsorption column selectively binds plasmid DNA in the solution under high salt and low pH, and then removes impurities and other bacterial components through buffer WB. Finally, the pure plasmid DNA is eluted from the silicon matrix membrane by buffer EB with low salt and high pH.

Features

1. The silicon matrix membranes in the centrifugal adsorption column are all specially made adsorption membranes imported from world-famous companies, with minimal difference in adsorption capacity between columns and good repeatability. It overcomes the disadvantage of unstable quality of domestic kit membrane.
2. There is no need to use toxic phenol, chloroform and other reagents, nor ethanol precipitation. It is fast and convenient. 0.5-2mg of pure high copy plasmid DNA can be rapidly extracted from 150-300 ml of Escherichia coli lb (Luria BERTANI) culture solution, and the extraction rate is 80-90%.
3. The unique process formula can remove endotoxin, and the endotoxin content is very low (<0.1eu/μg DNA), and the cell transfection effect was excellent. It can also be directly used in enzyme digestion, transformation, PCR, in vitro transcription, sequencing, and other molecular biology experiments.

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Product Number: PLK1101

Shipping and Storage

1. When used for the first time, add all RNase A carried by the kit to Buffer P1 (final concentration 100μg/ml) were stored at 4℃. If RNase A is inactivated in Buffer P1, the extracted plasmid may have trace RNA residue. Add RNase A to Buffer P1.
2. When the ambient temperature is low, SDS in Buffer P2 may precipitate and appear turbid or precipitated. It can be heated in a 37℃ water bath for a few minutes to restore clarity. Do not shake violently to avoid excessive foam formation.
3. Avoid volatilization, oxidation and pH change of reagents exposed to air for a long time, and close the cover of each solution in time after use.

Components

Description

This kit uses an improved SDS alkaline lysis method to lyse cells. The silicon matrix membrane in the centrifugal adsorption column selectively binds plasmid DNA in the solution under high salt and low pH conditions, and then removes impurities and other bacterial components through protein solution and Buffer WB. Finally, Buffer EB with low salt and high pH elutes pure plasmid DNA from the silicon matrix membrane.

Features

1. The silicon matrix membranes in the centrifugal adsorption column are all specially made adsorption membranes imported from world-famous companies, with minimal difference in adsorption capacity between columns and good repeatability. It overcomes the disadvantage of unstable quality of domestic kit membrane.
2. There is no need to use toxic phenol, chloroform and other reagents, nor ethanol precipitation. It is fast and convenient. 0.5-2mg of pure high copy plasmid DNA can be rapidly extracted from 150-300ml of Escherichia coli lb (Luria BERTANI) culture solution, and the extraction rate is more than 80%.
3. The obtained plasmid has high yield, high proportion of supercoiles and good purity, and can be directly used in various molecular biology experiments such as enzyme digestion, transformation, PCR, in vitro transcription, sequencing, etc.

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Product Number: PLK1001

Shipping and Storage

1. when used for the first time, add all RNase A carried by the kit to buffer P1 (final concentration 100μg/ml) were stored at 2-8℃. If RNase A is inactivated in buffer P1, the extracted plasmid may have trace RNA residue. Add RNase A to buffer P1.
2. when the ambient temperature is low, SDS in buffer P2 may precipitate turbidity or precipitation. It can be heated in a 37℃ water bath for a few minutes to restore clarity. Do not shake violently to avoid excessive foam formation.
3. avoid volatilization, oxidation and pH change of reagents exposed to air for a long time, and close the cover of each solution in time after use.
4. Buffer Er is transported at room temperature. It can be stored at 4℃ for one month, and stored at -20℃ for a long time.

Components

Description

This kit uses an improved SDS alkaline lysis method to lyse cells. Endotoxin is removed by a unique buffer Er selective combination with centrifugation. Then the silicon matrix membrane in the centrifugal adsorption column selectively binds plasmid DNA in the solution under high salt and low pH conditions. Impurities and other bacterial components are removed by buffer PE and buffer WB. Finally, the pure plasmid DNA is eluted from the silicon matrix membrane by buffer EB with low salt and high pH.

Features

1. The silicon matrix membranes in the centrifugal adsorption column are all specially made adsorption membranes imported from world-famous companies, with minimal difference in adsorption capacity between columns and good repeatability. It overcomes the disadvantage of unstable quality of domestic kit membrane.
2. The unique process formula can remove endotoxin, and the endotoxin content is very low (<0.1eu/μg DNA), and the cell transfection effect was excellent. It can also be directly used in enzyme digestion, transformation, PCR, in vitro transcription, sequencing, and other molecular biology experiments.

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Product Number: PLK0801

Shipping and Storage

1. When using for the first time, add all RNase A carried by the reagent kit to Buffer P1 (concentration 100μg/ml) and store at 4℃. If RNase A is inactivated in Buffer P1, the extracted plasmid may contain trace amounts of RNA residue. Adding RNase A to Buffer P1 is sufficient.
2. Buffer ER can be stored at 4℃ for one month. If it needs to be stored for a long time, it is recommended to store it at -20℃! When the ambient temperature is low, SDS in Buffer P2 may precipitate and become turbid or precipitate. It can be heated in a 37℃ water bath for a few minutes to restore clarity. Do not shake violently to avoid excessive foam formation.
3. To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.

Components

Description

This reagent kit extracts plasmid DNA from cultured bacteria using alkaline lysis method, using a unique solution formula and Buffer ER. It only requires a few simple centrifugations to remove impurities such as proteins, polysaccharides, endotoxins, RNA, etc., to obtain high-quality plasmid DNA. The OD260/280 of purified DNA is usually around 1.8, and the resulting plasmid can be directly applied in tasks that require high DNA purity, such as cell transfection and even animal in vivo experiments. The purification process in the later stage is operated in a 1.5ml small centrifuge tube, which is simple, does not require special equipment, does not require column passing, and does not require phenol chloroform extraction; Plasmids released by bacterial lysis can be completely recovered without worrying about the loss of plasmid DNA. This method extracts and purifies plasmid DNA with minimal damage to plasmids. Even large plasmids or ultra large BAC/PAC plasmids with a size of 10kb or even 100kb can be effectively purified as long as they can be extracted by alkaline lysis. You can choose to dissolve plasmids in any small volume, with a concentration of up to 5μg/μl. The super helix ratio can reach up to 95%, with no endotoxins and good transfection effect.

Features

1. No toxic reagents such as phenol and chloroform are needed, and no ethanol precipitation is required. Fast and convenient extraction of 150μg-600μg pure high copy plasmid DNA from 50-70ml of Escherichia coli LB (Luria Bertani) culture medium, with an extraction rate of 80-90%.
2. The obtained plasmids have high yield, concentration, super helix ratio, and purity, and can be directly used for various molecular biology experiments such as enzyme digestion, transformation, PCR, in vitro transcription, sequencing, etc.
3. The endotoxin content is extremely low (<0.1EU/μg DNA) and can be directly applied to cell transfection.

Application

Suitable for medium to high purity or transfection level plasmid preparation and BAC/PAC large-scale plasmid preparation

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