Manual

Product Number: RNK0101

Shipping and Storage

Trizoe can be stably stored at room temperature for 12 months. Nevertheless, for optimal results, we recommend storing in an environment of 2-8°C. 

Important Note

This product contains phenol, which is toxic and corrosive. If inhaled, in contact with the skin, swallowed, etc., it can cause poisoning, burns, and other bodily injuries. When using this product, protective equipment such as protective clothing, gloves, eye masks, face shields, etc. should be worn. If accidentally touched, immediately rinse with plenty of water and seek medical treatment.

Description

Trizoe Reagent is a broad-spectrum total RNA extraction reagent. The experimental operation is fast and convenient, with bright colors and easy layering. This reagent has a wide range of applications and can extract total RNA from animal tissues, plant materials, various microorganisms, and cultured cells. This method has good separation effects on small amounts of tissues (50-100mg) and cells (5×10⁶), as well as large amounts of tissues (≥ 1g) and cells (>107). The sample can be fully lysed in TRIpure while maximizing the integrity of RNA. After centrifugation with chloroform, the solution will be divided into three layers: the upper colorless aqueous phase, the middle layer, and the lower red organic phase, with RNA distributed in the supernatant layer. After collecting the supernatant, total RNA can be recovered by precipitation with isopropanol. The extracted total RNA has good integrity, no protein or DNA contamination, and can be used for various routine molecular biology experiments, such as RT-PCR Real-time RT-PCR、Northern blot、Dot Blot、 External translation, etc.

Trizoe reagent can promote the precipitation of various RNAs of different species and molecular weights. For example, RNA agarose gel electrophoresis extracted from rat liver and stained with ethidium bromide showed many discontinuous high molecular weight bands (mRNA and hnRNA components) between 7 kb and 15 kb, two dominant ribosomes~5 kb (28S) and~2 kb (18S), and low molecular weight RNA between 0.1 and 0.3 kb (tRNA, 5S). When the extracted RNA is diluted with TE, its A260/A280 ratio is ≥ 1.8. Note that for ordinary agarose gel electrophoresis, the position of 28S is about 2kb, and 18S is about 1kb. The position of gel with different concentrations varies greatly.

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Product Number: PDA-250g

Product details

Formula of culture medium (per liter); pH5.6 ± 0.2(25℃)

Instructions

Weigh 46.0g of this product into 1L of distilled water or deionized water, dissolve at a low temperature, divide and sterilize at 115℃ for 30 minutes. After sterilization, shake well to prevent the agar from settling at the bottom of the vessel and solidifying. Cool to around 50℃, pour into a petri dish, and set aside.

Application

Used for counting mold and yeast.

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Product Number: RNK4501LY

Shipping and Storage

Freeze dried powder is stored at 4 ℃, and after reconstitution, the solution is stored at -20 ℃

Components

Description

DNase I is a deoxyribonuclease that requires a divalent cation and can be used to degrade single stranded or double stranded DNA. The principle is that DNase I hydrolyzes phosphate diester bonds to produce single nucleotides or oligonucleotides with 5 '- phosphate groups and 3' - OH groups. Both Mg²⁺ and Mn²⁺ can activate the activity of DNase I, while Ca²⁺ concentration directly affects enzyme activity. When Mg²⁺ is present, it can randomly generate incisions on each single strand of double stranded DNA; In the presence of Mn²⁺, double stranded DNA can be broken and fragmented. Used for the preparation of RNA without DNA contamination, reverse transcription, and in vitro transcription experiments.

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Product Number:DRK21

Shipping and Storage

Room temperature (4-30 ℃)

Components

Description

This product is an efficient RNase inactivation and DNA cleaning agent. It contains multiple components that can efficiently inactivate RNase and DNA contamination on glass and plastic surfaces, as well as in the air, ensuring a clean working environment. Containers, laboratory tables, gun heads, pipettes, electrophoresis tanks, etc. that have been cleaned with RNase and DNA scavengers can ensure that they are free of RNase and DNA contamination.

Preparation and important precautions before the experiment

1. RNase and DNA scavengers can be used directly, do not dilute them, as dilution will reduce their inactivation efficacy.
2. Operators should wear gloves during the use of RNase and DNA scavengers to avoid direct skin contact. When using spray bottles, please wear a mask and carry out in a ventilated environment such as a fume hood to prevent irritation to people.

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Product Number: RNK5401

Shipping and Storage

1. Inappropriate storage at low temperatures (4℃ or -20℃) can cause solution precipitation, affecting the effectiveness of use. Therefore, transportation and storage are carried out at room temperature (15℃ -25℃).
2. To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.

Components

Description

Bone tissue is hard, bone cell density is low, and the peripheral matrix contains a large amount of mucins (proteoglycans) and RNA, which are difficult to separate and cannot be high-quality extracted using the traditional Trizol method. This reagent kit uses a unique phenol/chloroform free lysis solution and adds multiple components to remove bone tissue proteoglycans. Meanwhile, the unique genomic DNA clearance column technology can effectively remove gDNA residues, and the obtained RNA generally does not require DNase digestion and can be used for reverse transcription PCR, fluorescence quantitative PCR, and other experiments. The unique Buffer CLB and Buffer RLT Plus rapidly lyse cells and inactivate cell RNA enzymes, centrifuge precipitate to remove polysaccharides and secondary metabolites, then lyse the mixture and regulate RNA binding with ethanol to adsorb onto the genomic DNA clearance column. RNA is selectively washed and filtered, and residual DNA adsorbed on the genomic DNA clearance column cannot be washed off and discarded along with the column to remove DNA. After adjusting the binding conditions with ethanol, the filtered RNA selectively adsorbs onto the silica matrix membrane in a highly dissociated salt state. Through a series of rapid rinsing centrifugation steps, Buffer RW1 and Buffer RW remove impurities such as cell metabolites and proteins. Finally, low salt RNase free H₂O washes the pure RNA off the silica matrix membrane.

Features

1. No toxic reagents such as phenol and chloroform are used, and no steps such as ethanol precipitation are required.
2. Simplicity, single sample operation can generally be completed within 35 minutes, making it the simplest and fastest reagent kit in the world.
3. The independently developed genomic DNA clearance column technology can effectively remove gDNA residues, and the obtained RNA generally does not require DNase digestion and can be used for experiments such as reverse transcription PCR and fluorescence quantitative PCR.
4. Widely adaptable, it can extract various bone tissues, including mineralized bone tissue.
5. Multiple column washes ensure high purity, with a typical OD260/OD280 ratio of 1.9~2.2 and almost no DNA residue. It can be used for RT-PCR, Northem blot, second-generation sequencing, and various experiments.

Application

Suitable for rapid extraction of total RNA from bone tissue cells, unique genomic DNA clearance column technology can effectively remove visible gDNA residues on electrophoresis. RNA can be used for reverse transcription PCR, fluorescence quantitative PCR, etc.

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Product Number: RNK5301

Shipping and Storage

1. Inappropriate storage at low temperatures (4℃ or -20℃) can cause solution precipitation, affecting the effectiveness of use. Therefore, transportation and storage are carried out at room temperature (15℃ -25℃).
2. To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to air, each solution should be covered tightly in a timely manner after use

Components

Description

On the basis of our company's exclusive introduction of EASYspin phenol free and chloroform based rapid RNA extraction technology, we have also independently developed the genome DNA clearance column technology, which can effectively remove gDNA residues. The obtained RNA generally does not require DNase digestion and can be used for reverse transcription PCR, fluorescence quantitative PCR and other experiments. The unique Buffer CLB and Buffer RLT Plus rapidly lyse cells and inactivate cell RNA enzymes. Centrifuge precipitation is used to remove polysaccharides, polyphenols, and secondary metabolites. Then, the mixture is lysed and ethanol is used to regulate RNA binding and adsorption onto the genomic DNA clearance column. RNA is selectively washed and filtered, and the residual DNA adsorbed on the genomic DNA clearance column cannot be washed off. The column is discarded together to remove DNA. After adjusting the binding conditions with ethanol, the filtered RNA selectively adsorbs onto the silica matrix membrane in a highly dissociated salt state. Through a series of rapid rinsing centrifugation steps, Buffer RW1 and Buffer RW remove impurities such as cell metabolites and proteins. Finally, low salt RNase free H2O washes the pure RNA off the silica matrix membrane.

Features

1. No toxic reagents such as phenol and chloroform are used, and no steps such as ethanol precipitation are required.
2. Simplicity, single sample operation can generally be completed within 25 minutes, making it the simplest and fastest reagent kit in the world.
3. The independently developed genomic DNA clearance column technology can effectively remove gDNA residues, and the obtained RNA generally does not require DNase digestion and can be used for experiments such as reverse transcription PCR and fluorescence quantitative PCR.
4. The world's leading adaptability is extremely extensive, and it can extract hundreds of failed samples from complex Chinese herbs such as dendrobium/salvia miltiorrhiza/snow lotus/ginseng, complex starch seeds such as rice/wheat/corn seeds, complex fruits such as grapes/blueberries/strawberries/watermelon fruits, complex stress resistant plants such as holly/pine needles/sea buckthorn/Populus euphratica, complex flowers such as roses/plums/peonies, complex polysaccharide plants such as seaweed/cactus/aloe vera, lily bulbs/rice seeds, etc. The EASYspin Plus complex Plant RNA Kit has published over 150 articles. For a detailed sample list, please refer to the company's homepage product introduction or contact us to request over 150 original published articles.
5. Multiple column washes ensure high purity, with a typical OD260/OD280 ratio of 2.1~2.2 and almost no DNA residue. It can be used for RT-PCR, Northern blot, second-generation sequencing, and various experiments.

Application

Suitable for rapid extraction of total RNA from plant tissue cells, unique genomic DNA clearance column technology can effectively remove visible gDNA residues on electrophoresis. RNA can be used for reverse transcription PCR, fluorescence quantitative PCR, etc.

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Product Number: RNK5201

Shipping and Storage

1. Improper storage of room temperature components at low temperatures (4℃ or-20℃) can cause solution precipitation and affect the effectiveness of use. Therefore, transportation and storage are carried out at room temperature (15℃ -25℃).
2. To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.

Components

Description

The unique Buffer RPA rapidly cleaves cells and inactivates cellular RNA enzymes. After adjusting the binding conditions with ethanol, RNA selectively adsorbs onto the silica matrix membrane in a highly dissociated salt state. DNase directly digests residual DNA on the column, and then through a series of rapid rinsing centrifugation steps, Buffer RW1 and Buffer RW remove impurities such as cell metabolites and proteins. Finally, low salt RNase free H₂O washes pure RNA off the silica matrix membrane.

Features

1. Completely do not use toxic substances β-Mercaptoethanol/phenol/chloroform does not require steps such as ethanol precipitation.
2. Simplicity, single sample operation can generally be completed within 40 minutes, making it the simplest and fastest reagent kit in the world.
3. The RNA obtained through DNase I column digestion without residual DNase can be directly used for reverse transcription fluorescence quantitative PCR, second-generation sequencing, chip, RACE and other experiments.
4. World leading, it is the most widely adaptable reagent kit among similar products, which can extract plants including rice, corn, wheat, Arabidopsis, tomato, tobacco, and general polysaccharides and polyphenols such as cotton and holly.
5. Multiple column washes ensure high purity, with a typical OD260/OD280 ratio of 2.0-2.2 and no DNA residue. It can be directly used for fluorescence quantitative PCR, RT-PCR, chips, second-generation sequencing, Northern blot and other experiments.

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Product Number: RNK5001

Shipping and Storage

Room temperature (15-30℃).

Components

Note: All reagents, when store in indicated temperature, are stable for 9 months.

Description

RNAstore Blood RNA Kit is designed for isolation of total RNA from blood samples stored in RNA fixer(Same as RNA later) Solution. Blood samples can be processed immediately, or they can be stored in RNA fixer Solution(provided with the kit) for a few days at ambient temperature or for prolonged periods at -20℃ prior to RNA extraction. The Kit RNA isolation procedure consists of two parts:

1. Cell lysis in a guanidinium-based solution and initial purification of the RNA by phenol/chloroform extraction
2. Final RNA purification by solid-phase extraction on a glass fiber filter. This procedure completely removes contaminants and enzyme inhibitors producing high-quality RNA. RNA purified using the Kit is ready for applications such as qRT-PCR.

Materials and Equipment to be Supplied by User

1. Microcentrifuge capable of 13,000×g
2. 100% ethanol, water-saturated-Phenol: Chloroform( 5:1)
3. RNase-free filter pipette tips
4. Blood collection tubes (recommended anticoagulant: potassium EDTA or sodium EDTA)
5. 1.5 or 2.0ml microcentrifuge tubes
6. Shaking incubators or heat blocks capable of 75°C-90°C

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Product Number: RNK4901

Shipping and Storage

Room temperature (15-30℃).

Components

Note:1)Proteinase K is a lyophilizate. Centrifuge a few seconds and reconstitute with 1 ml distilled water, aliquot solution. Store at –20°C. Avoid repeated freezing and thawing.

2)All reagents, when store in indicated temperature, are stable for 9 months.

Description

PAX Blood RNA Kit is designed for isolation of total RNA from blood samples stored in preservation reagents and PAXgene® Blood RNA Tubes. This procedure completely removes contaminants and enzyme inhibitors producing high-quality RNA. RNA purified using the PAx Blood RNA Kit is ready for applications such as RT-PCR.

The samples are removed from the preservation reagents. For blood samples stored in PAXgene® Blood RNA Tubes, the cells are collected by centrifugation. Samples are washed and lysed under an optimized buffer containing Proteinase K. The samples are centrifuged to remove cell debris and other particulates. After adjusting the binding conditions with ethanol, the samples are loaded on the RNA binding column. With a brief centrifugation or vacuum step, the samples pass through the column matrix which binds the RNA. Genomic DNA is removed with an on-the-column DNase l digestion treatment. After three wash steps, purified RNA is eluted with RNase-free water.

Materials and Equipment to be Supplied by User

1. Microcentrifuge capable of 13,000×g
2. 100% isopropanol
3. RNase-free filter pipette tips
4. RNase-free water
5. 1.5 or 2.0 ml microcentrifuge tubes
6. Shaking incubators or heat blocks capable of 55°C, 65°C
7. Centrifuge with swing-bucket rotor capable of 5,500×g

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Product Number: RNK4501

Shipping and Storage

Store at -20℃.

Components

Description

DNase I is a deoxyribonucleic acid endonuclease that requires a divalent cation and can be used to degrade single or double stranded DNA. The principle is that DNase I hydrolyzes phosphate diester bonds to produce single or oligonucleotides with 5'- phosphate groups and 3'-OH. Both Mg²⁺ and Mn²⁺ can activate the activity of DNase I, and the concentration of Ca²⁺ directly affects the activity of the enzyme. When Mg²⁺ is present, random incisions can be generated on each single strand of double stranded DNA; In the presence of Mn²⁺ , double stranded DNA can be broken and fragmented. Used for the preparation, reverse transcription, and in vitro transcription experiments of RNA without DNA contamination.

Preparation and important precautions before the experiment

1. Because DNase I is often used in DNA digestion experiments that require maintaining RNA integrity, it minimizes RNase contamination during enzyme preparation and can be safely used for RNA extraction. However, since this enzyme does not contain RNase inhibitors, it is recommended to pay attention to preventing external RNase contamination during use.
2. DNase I is greatly affected by shear forces. Before use, the centrifuge tube can be inverted and mixed evenly to avoid vortex oscillation.
3. Do not exceed 1U of DNase I for every 1μg of RNA processed.

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