Product Number: P1331

Storage

Poly A RNA carrier is temperature stable, can ship at room temperature. Store at -20℃, Keep away from sunlight, sealed in dry place.

Description

Poly A is a synthetic nucleic acid sequence, which increase the yield of RNA or DNA during extraction process. Ethanol precipitation is commonly used to recover DNA and RNA from liquid samples. However, ethanol precipitation cannot completely recover the nucleic acid from the sample, at least 30% of the nucleic acid will lost. If the concentration of nucleic acid in the liquid sample is very low or DNA < 200bp, only 50% DNA and RNA can be recovered by ethanol precipitation. Poly A carrier is a type of synthetic polymer. Adding Poly A carrier in ethanol precipitation can significantly improve the yield of nucleic acid precipitation, and the recovery rate of trace DNA can reach 95-98%. At the same time, short primer (< 22bp) fragments and dNTP can be selectively removed for precipitation and recovery of labeled probes, and untagged dNTP can be removed. Compared to biologically derived nucleic acid precipitation aids such as glycogen and tRNA, Poly A carrier itself is free of nucleic acid pollution, DNase and RNase activity, and does not affect digestion, connection, transcription, PCR, transformation and transfection, nor does it affect DNA electrophoresis and DNA protein interaction. Poly A carrier has become the most commonly used nucleic acid precipitant.

Package

1g, 5g, or bulk.

Working concentration

A concentration of 0.5 mg/ml is recommended.

Features

1. Significantly improve the yield of DNA or RNA precipitation.

2. The recovery of trace DNA and RNA (20pg) was 95-98%.

3. It does not affect the enzyme cutting, linking, transcription, PCR and other reactions.

4. It does not affect the interaction between electrophoresis and DNA protein.

Note

Poly A carrier will increase the optical density value of RNA or DNA. Thus, when measuring the optical density value, to eliminate the influence of Poly A carrier, a blank control sample should be made according to the same experimental process (use the same reagent and Poly A carrier, but do not contain RNA or DNA samples, and dissolve the final Poly A carrier precipitation in the same solution as the sample). The optical density values of sample and blank control were measured at 260 and 280 nm. The actual optical density of the sample can be obtained by subtracting the optical density of the blank control from that of the sample. If the quantification does not need to be very accurate, it can also be estimated.

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100mM dNTP solution supplies by Bulk package.

100mM dNTPs solution,100mM dNTP set dATP dCTP dGTP dTTP 100mM each in 4 tubes.

Description

dNTP Set consists of 100 mM aqueous solutions at pH 7.0 of each of dATP, dCTP, dGTP and dTTP, each in a separate vial. Because the nucleotides are provided in separate solutions, dNTP sets offer maximum flexibility in the preparation of reaction mixes for different applications.

Applications

For use in all molecular biology applications, including PCR, long PCR, real-time PCR, high fidelity PCR, RT-PCR, cDNA synthesis, primer extension, DNA sequencing, and DNA labeling.

Quality Control

Functionally tested in PCR with Taq and Pfu DNA Polymerases.

Greater than 99% purity of each component was confirmed by HPLC.

Free of endodeoxyribonuclease, exodeoxyribonuclease, ribonuclease, and phosphatase activities. 

Storage

Long Term (Infrequent use; 1-2 times per month): -70degree.

Daily/Weekly use: -20degree.

Package

Bulk package.

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Feature

Greater than 99% purity was confirmed by HPLC.

Functionally tested in in vitro transcription.

Highly stable – the natural pH of nucleotide solutions ensures stability during long-term storage.

Applications

in vitro transcription.

RNA amplification.

siRNA synthesis.

aRNA synthesis.

Description

A convenient set of 100 mM solutions of each ATP, CTP, GTP, and UTP.

Concentration:100mM

Purity: >99% by HPLC

Storge: Store at -20℃

MOQ:1ml of each

Delivery time:2-3 working days after receiving the payment.

Payment method: T/T or Western Union or Money Gram full in advance.

OEM and Bulk both are available.

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Molecular Formula: C9H12N2Na3O14P3

Molecular Weight: 468.1 (free acid)

CAS Number 1173-82-6 (free acid)

Appearance: Clear Colorless solution

Concentration: 100mM±5% Purity (HPLC) ≥99%

λmax 267nm±2nm

Absorbance Rations :

A250/A260 0.73±0.03 (pH 7.0)

A280/A260 0.38±0.03 pH (22~25C) 7.0±0.1

Applications: For use in all molecular biology applications, including PCR, real-time PCR, and RT-PCR with Taq DNA Polymerase, cDNA synthesis, and primer extension.

Quality Control

Molecular Biology Grade

1. The concentration is verified by optical density spectrophotometry.

2. This preparation is free of DNase and RNase contamination as determined by incubation of the dUTP with radioactive substrates.

3. The purity of this preparation is determined by HPLC. The minimum passing specification for the triphosphate content is >99%.

4. This lot of dUTP has been performance tested with Taq DNA polymerase, Pfu DNA polymerase.

Storage

Long term (Infrequent use; 1-2 times per month): -70 C

Daily/Weekly use: -20 C

Always avoid freeze-thaw cycles or exposure to frequent temperature changes. These fluctuations can greatly alter product stability.

Note: the 100mM dUTP is not suitable for PCR with proofreading enzymes or enzyme mixes containing proofreading enzymes.

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Description

dNTP Mix 10mM each is a premixed solution containing sodium salts of dATP, dCTP, dGTP and dTTP, each at 10mM in water; the total concentration of nucleotides therefore is 40mM (pH 7.4).dNTP Mixes are ready-to-use solutions designed to save time and to provide higher reproducibility in PCR and other applications.

Applications

For use in all molecular biology applications, including PCR, long PCR, real-time PCR, high fidelity PCR, RT-PCR, cDNA synthesis, primer extension and DNA labeling.

Quality Control

Functionally tested in PCR with Taq and Pfu DNA Polymerases.

Greater than 99% purity of each component was confirmed by HPLC.

Free of endodeoxyribonuclease, exodeoxyribonuclease, ribonuclease, and phosphatase activities.

Storage

Long Term (Infrequent use; 1-2 times per month): -70degree

Daily/Weekly use: -20Cdegree

Guarantee 12 months

Package

500ml

OEM and Bulk both are available!

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Description

dNTP mix 10mM each is a premixed solution containing sodium salts of dATP, dCTP, dGTP and dTTP, each at 10mM in water; the total concentration of nucleotides therefore is 40mM (pH 7.4).dNTP Mixes are ready-to-use solutions designed to save time and to provide higher reproducibility in PCR and other applications.

Applications

For use in all molecular biology applications, including PCR, long PCR, real-time PCR, high fidelity PCR, RT-PCR, cDNA synthesis, primer extension, and DNA labeling.

Quality Control

Functionally tested in PCR with Taq and Pfu DNA Polymerases.

Greater than 99% purity of each component was confirmed by HPLC.

Free of endodeoxyribonuclease, exodeoxyribonuclease, ribonuclease, and phosphatase activities.

Storage

Long Term (Infrequent use; 1-2 times per month): -70℃

Daily/Weekly use: -20C℃

OEM and Bulk both are available.

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dNTP Mix 25mM each is a premixed solution containing sodium salts of dATP, dCTP, dGTP and dTTP, each at 25mM in water; the total concentration of nucleotides, is 100mM (pH 7.4).dNTP Mixes are ready-to-use solutions designed to save time and to provide higher reproducibility in PCR and other applications.

Applications

For use in all molecular biology applications, including PCR, long PCR, real-time PCR, high fidelity PCR, RT-PCR, cDNA synthesis, primer extension, and DNA labeling.

Quality Control

 Functionally tested in PCR with Taq and Pfu DNA Polymerases.

 Greater than 99% purity of each component was confirmed by HPLC.

 Free of endodeoxyribonuclease, exodeoxyribonuclease, ribonuclease, and phosphatase activities.

Storage

Long Term (Infrequent use; 1-2 times per month): -70℃

Daily/Weekly use: -20C℃

OEM and Bulk both are available

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This dNTP/dUTP mix is manufactured by MBS, one of the few primary manufacturers in the industry. The dUTP/dNTP mix is 10mM each, which containing sodium salts of dATP, dCTP, dGTP and dUTP, each at 10mM in water. So the total concentration of nucleotide is 40mM. For direct use in PCR with Taq DNA Polymerase, reverse transcription, and other applications.

Quality Control

Functionally tested in PCR with Taq DNA Polymerase.

Greater than 99% purity of each component was confirmed by HPLC.

Free of human and E.coli DNA.

Free of endo-, exodeoxyribonuclease, ribonuclease, phosphatase, and nicking activities.

Applications

For use in all molecular biology applications including PCR, real-time PCR & RT-PCR with Taq DNA Polymerase, cDNA synthesis, MDA, and DNA sequencing.

Note

dNTP/dUTP mix is not suitable for PCR with proofreading enzymes or enzyme mixes containing proofreading enzymes.

Storage

Store at -20°C.

OEM and Bulk both are available

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2'-F-dATP (2'-Fluoro-2'-Deoxyadenosine-5'-Triphosphate, Sodium Salt)

2'-F-dCTP (2'-Fluoro-2'-Deoxycytidine-5'-Triphosphate, Sodium Salt)

2'-F-dGTP (2'-Fluoro-2'-Deoxyguanosine-5'-Triphosphate, Sodium Salt)

2'-F-dUTP (2'-Fluoro-2'-Deoxyuridine-5'-Triphosphate, Sodium Salt)

Description:

The 2'F-dNTP(2'-Fluorine-dNTP) we supplied is the 100mM soulution of 2'F-dATP, 2'F-dCTP, 2'F-dGTP, and 2'F-dUTP in sepreate tubes.

Concentration: 100mM

Purity: >98% by HPLC

Storge: Store at -20℃

MOQ: 1ml of each

Delivery time: 2-3 working days after receiving the payment.

Payment method: T/T or Western Union or Money Gram full in advance.

OEM and Bulk both are available.

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