BFE – MEBEP Bio Science https://www.mebep.com make experiment be predigest Mon, 21 Jul 2025 01:39:58 +0000 en-US hourly 1 https://wordpress.org/?v=6.9.4 bovine fibronectin ELISA kit https://www.mebep.com/elisa-kit/bovine-fibronectin-elisa-kit/ Mon, 21 Jul 2025 01:39:57 +0000 https://mebep.com/?p=3180 Manual

Product Number: BFE-48T

Shipping and Storage

  1. Storage: 2-8℃.
  2. Validity: six months.

Component

Component48T
User manual1
Closure plate membrane2
Sealed bags1
Microelisa stripplate1
Standard0.3mL×6 bottle
HRP-Conjugate reagent5mL×1 bottle
Sample diluent3mL×1 bottle
Chromogen Solution A3mL×1 bottle
Chromogen Solution B3mL×1 bottle
Stop Solution3mL×1 bottle
20×Wash solution15mL×1 bottle

Note: Standard concentration was followed by: 100、50、25、12.5、6.25、0ug/mL.

Description

The kit assay Bovine FN level in the sample, use Purified Bovine FN antibody to coat microtiter plate wells, make solid-phase antibody, then add FN to the wells, Combined antibody which With HRP labeled, become antibody-antigen-enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of FN in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Application

This kit allows for the determination of FN concentrations in Bovine serum, plasma, tissue homogenates and other biological fluids.

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