Product Number: DNK1301

Shipping and Storage

1. When the ambient temperature is low, some detergent ingredients in Buffer NLF will precipitate and become turbid or precipitate. It can be heated in a 37°C water bath for a few minutes, and then gently shaken to restore clarity. Do not shake violently to avoid excessive foam formation.
2. Buffer PP may experience precipitation and precipitation, and can be re dissolved in a water bath at 37°C for a few minutes. If it cannot be completely dissolved, it will not affect the effectiveness of use. Simply take the upper solution.
3. To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.

Components

Description

This kit is used for rapid extraction of genomic DNA from various bacteria. Bacterial samples are added to Buffer NLF (or lysozyme or other alcohols to aid in cell wall lysis). Firstly, the cells are lysed under the action of strong detergents to release genomic DNA. Then, RNase A is added to remove RNA, followed by selective precipitation of buffer PP to remove protein. Finally, pure genomic DNA is precipitated in isopropanol and re dissolved in DNA solution.

Features

1. No need to use toxic reagents such as phenol.
2. Fast and simple, the operation of a single sample can generally be completed within 30 minutes.
3. The results are stable, with high yield. The typical OD260/OD280 ratio is 1.7~1.9, and the length can reach 50kb-150kb. It can be directly used for library construction, PCR, Southern blot, and various enzyme digestion reactions.

Application

Used for rapid extraction of various bacterial genomic DNA

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