DNK49 – MEBEP Bio Science https://www.mebep.com make experiment be predigest Wed, 23 Jul 2025 09:20:03 +0000 en-US hourly 1 https://wordpress.org/?v=6.9.4 Blood Mitochondrial DNA Extraction Kit https://www.mebep.com/nucleic-acid-isolation/blood-mitochondrial-dna-extraction-kit/ Wed, 09 Jul 2025 08:45:00 +0000 https://mebep.com/?p=3144 Manual

Product Number: DNK49

Shipping and Storage

Transport and store at room temperature, with a shelf life of one year.

Components

Component50TPackage
10×Blood Lysis Buffer15mL15mL natural color bottle
Frozen Blood Lysis Buffer250mL×2250mL natural color bottle
White Cell Wash Buffer100mL125mLnatural color bottle
Buffer A7.5mL10mLnatural color bottle
Buffer B15mL15mLBrown glass bottle
Buffer C12mL15mLnatural color bottle
Buffer P50mL60mLBrown glass bottle
BufferCarrier  (IPA type)50mL60mL natural color bottle
Buffer EB1mL10mL natural color bottle

Description

This product is developed based on our company's blood DNA extraction product and is specifically used as a reagent for extracting mitochondrial DNA (mtDNA) from fresh or frozen human anticoagulant whole blood. The classification of cells in human blood is as follows: the ratio of red blood cells to white blood cells in human blood is 1000-6000:1, but there is no DNA in red blood cells, so the mitochondrial DNA mainly comes from white blood cells. White blood cells are divided into two types: polymorphonuclear leukocytes (PMNs) and peripheral blood mononuclear cells (PBMCs). The former includes neutrophils, which account for 50-70% of the total white blood cells, eosinophils, which account for 0.5-5%, and basophils, which account for 0-1%. The latter includes lymphocytes (T cells, B cells, and NK cells) that account for 20-40% of the total white blood cell count, monocytes (1-8%), and dendritic cells (0.3-0.9%).

Features

  1. High DNA yield. If the sample is fresh blood, the highest mitochondrial DNA yield can reach 1μg/mL fresh blood. If the sample is frozen blood, the difference in DNA yield is significant.
  2. The DNA is pure, with OD260/OD280 between 1.8-2.0.
  3. It can be directly used for PCR, enzyme digestion, hybridization and other experiments.
  4. Applicable to mammalian blood, not applicable to blood of other animals.
  5. This product is sufficient for 50 uses and can handle 3mL of anticoagulant blood each time.
  6. Each 1E7 white blood cell can be purified to 40-80ng mtDNA.
  7. This product can only be used for scientific research.

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