DNK51 – MEBEP Bio Science https://www.mebep.com make experiment be predigest Wed, 23 Jul 2025 09:20:12 +0000 en-US hourly 1 https://wordpress.org/?v=6.9.4 Blood Mitochondrial DNA Extraction Kit (Enzyme) https://www.mebep.com/nucleic-acid-isolation/blood-mitochondrial-dna-extraction-kit-enzyme/ Wed, 09 Jul 2025 08:48:53 +0000 https://mebep.com/?p=3147 Manual

Product Number: DNK51

Shipping and Storage

2-8℃, among which DNase I and RNase A need to be stored at -20℃ with a shelf life of one year.

Components

Component50T100T
DNase I12mg2×12mg
RNase A500μL2×500μL
10×Blood lysis buffer100mL2×100mL
Cell lysis buffer50mL100mL
Mt Wash buffer25mL50mL
DNAase reaction buffer6mL12mL
Mt lysis buffer10mL20mL
Buffer PP7.5mL15mL
Poly Carrier0.5mL1mL
Buffer TE25mL50mL

Description

This kit can be used to isolate complete and purified mitochondrial DNA from blood. The key to extracting mitochondrial DNA is to remove nuclear DNA as much as possible. This reagent kit uses differential centrifugation to obtain relatively pure mitochondria, and then uses multiple steps such as DNA enzyme digestion and lysis buffer system to remove nuclear DNA, finally obtaining pure mitochondrial DNA. Can be used for experiments such as PCR that require high purity.

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