DTK806 – MEBEP Bio Science https://www.mebep.com make experiment be predigest Wed, 23 Jul 2025 09:17:00 +0000 en-US hourly 1 https://wordpress.org/?v=6.9.4 Monkeypox virus nucleic acid detection kit/with internal standard (fluorescence PCR method) https://www.mebep.com/pcr-detection-kit/monkeypox-virus-nucleic-acid-detection-kit-with-internal-standard-fluorescence-pcr-method/ Fri, 11 Jul 2025 01:53:28 +0000 https://mebep.com/?p=3166 Manual

Product Number: DTK806

Shipping and Storage

  1. Store in the dark at -20℃± 5℃, with a shelf life of 12 months.
  2. The transportation mode of foam box and ice bag shall be adopted, and the transportation shall not exceed 4 days; Store in the dark at -20 ± 5℃ after opening; Repeated freezing and thawing should not exceed 6 times.
  3. Production date and expiration date: see outer packaging box.

Component

Component25T50TMain components
qRT-PCR Pre mixed solution (containing enzymes)400μL800μLTris, KCl, MgCl2, dNTPs, Taq enzyme, UNG enzyme, etc.
Primer probe MPV (including IC)100μL200μLPrimer probe
Positive control MPV (including IC)500μL500μLPlasmids containing target detection gene fragments.
Negative control500μL500μLNormal saline.

Description

This reagent kit is designed based on the principle of fluorescence PCR technology, with specific primers and Taqman probes designed for monkeypox virus (MPXV). It is detected by a fluorescence PCR detector to achieve the detection of monkeypox virus (MPXV) nucleic acid. The target gene in the reagent is a conserved gene in humans.

Application

This kit is used for qualitative detection of monkeypox virus (MPV) nucleic acid and for auxiliary diagnosis and epidemiological monitoring of monkeypox virus (MPV) infection.

Specimen collection

  1. Sample types: Blood (collected fresh anticoagulant) (non heparin), serum, vesicles or pustules, rash exudate.
  2. Storage conditions: The collected specimens should be sent for testing in a timely manner. Those tested within 24 hours should be stored at 4℃, and those that exceed 24 hours should be stored at -70℃ (if there is no -70℃ storage condition, they should be temporarily stored at -20 ± 5℃), and repeated freezing and thawing should be avoided.

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