DTK812 – MEBEP Bio Science https://www.mebep.com make experiment be predigest Wed, 23 Jul 2025 09:17:23 +0000 en-US hourly 1 https://wordpress.org/?v=6.9.4 Herpes simplex virus type I / type II nucleic acid detection kit (double fluorescence PCR method) https://www.mebep.com/pcr-detection-kit/herpes-simplex-virus-type-i-type-ii-nucleic-acid-detection-kit-double-fluorescence-pcr-method/ Mon, 21 Jul 2025 01:44:18 +0000 https://mebep.com/?p=3182 Manual

Product Number: DTK812

Shipping and Storage

  1. Store at -20℃ away from light, with a shelf life of 12 months.
  2. Low temperature transportation cannot exceed 4 days; After opening, store in the dark at -20℃ without affecting the expiration date. Avoid repeated freeze-thaw cycles, as six freeze-thaw cycles will not affect the detection results.
  3. Production date and expiration date: see outer packaging box.

Component

Component25T25TMain components
qPCR premix (containing enzymes)400μL800μLTris, KCl, MgCl2, dNTPs, Taq enzymes, etc
Primer probe HSV1/HSV2100μL200μLPrimer probe
Positive control HSV1/HSV250μL50μLPlasmids containing target detection gene fragments
Negative control50μL50μLWater treated with diethyl carbonate

Description

This reagent kit is designed based on the principle of fluorescence PCR technology, with specific primers and Taqman probes designed for herpes simplex virus type I/II. It is detected by a fluorescence PCR detector to achieve the detection of herpes simplex virus type I/II nucleic acid.

Application

This kit is used for qualitative detection of herpes simplex virus type I/II (HSV1/HSV2) nucleic acid, and is used for auxiliary diagnosis and epidemiological monitoring of herpes simplex virus type I/II infection.

Specimen collection

  1. Sample types: herpetic fluid, saliva, throat wash, corneal swabs, secretions, cerebrospinal fluid, and other samples.
  2. Storage conditions: The collected specimens should be sent for testing in a timely manner. Those tested within 24 hours should be stored at 4℃, and those tested beyond 24 hours should be stored at -70℃, avoiding repeated freezing and thawing.

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