EK0630 – MEBEP Bio Science https://www.mebep.com make experiment be predigest Wed, 19 Nov 2025 09:54:17 +0000 en-US hourly 1 https://wordpress.org/?v=6.9.4 One Step Seamless Cloning Mix (kit) https://www.mebep.com/other/one-step-seamless-cloning-mix-kit/ Wed, 19 Nov 2025 09:53:38 +0000 https://mebep.com/?p=3484 Manual

Product Number: EK0630

Shipping and Storage

Store at -20℃.

Component

ComponentEK0630
2×Cloning MasterMix100µL
PUC19 Vector, Linearized(20ng/µL)10µL
500 bp Control Insert (20ng/µL)10µL
ddH2O1mL

Description

Seamless cloning technology is a simple, efficient, and rapid DNA cloning method that enables directional insertion of DNA fragments into any site of a vector. This product does not rely on T4 ligase and is not restricted by restriction enzyme sites of the vector or target fragments. Instead, it directly employs homologous recombination through a specialized enzyme, allowing directional recombination between linearized vectors and PCR fragments with 20-25bp overlapping regions at both ends of the linearized vector. A 5-minute to 15-minute reaction at 50°C can rapidly achieve directional seamless cloning of 1-5 fragments.

EK0630, One Step Seamless Cloning Mix single segment
Schematic diagram of seamless connection principle for single segment
EK0630, One Step Seamless Cloning Mix multiple segments
Schematic diagram of seamless connection principle for multiple segments

Features

  1. Within 15 minutes, one or more long or short PCR amplification fragments (flat/A end) can be inserted into the vector.
  2. Cloning can be performed at any site without being limited by the availability of the vector and insertion site, as well as the flat/sticky ends.
  3. Seamless cloning, insertion points will not introduce unwanted base sequences.
  4. Efficient and accurate, with a gram-positive rate of over 95%.

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