ELK010 – MEBEP Bio Science https://www.mebep.com make experiment be predigest Thu, 11 Dec 2025 03:24:01 +0000 en-US hourly 1 https://wordpress.org/?v=6.9.4 Adalimumab trough levels ELISA Kit https://www.mebep.com/elisa-kit/adalimumab-trough-levels-elisa-kit/ Thu, 11 Dec 2025 03:24:00 +0000 https://mebep.com/?p=3525 Manual

Product Number: ELK010

Shipping and Storage

  1. Store the kit at 2~8°C. Do not freeze.
  2. Unused microplate strips should be sealed and stored dry.

Component

Component96T
Pre-coated microplate (breakable strips) with Adalimumab conjugate antigen1 plate(12 holes×8 strips)
Adalimumab Standard solutions6 bottles (1ml/bottle)
Anti-Adalimumab Antibody Enzyme Conjugate6mL
Chromogen Solution A6mL
Chromogen Solution B6mL
Stop Solution6mL
Sample Diluent(10×, 6mL)
Concentrated Wash Buffer(20×, 6mL)

Description

This kit employs a competitive ELISA method. The microplate is pre-coated with Adalimumab conjugate antigen. Adalimumab standards or samples are added. Free Adalimumab and the Adalimumab conjugate antigen pre-coated on the microplate wells compete for the anti-Adalimumab antibody enzyme conjugate. After adding TMB substrate for color development and then stop solution, the color changes from blue to yellow. The absorbance is measured at 450 nm using a microplate reader. The absorbance value is inversely proportional to the Adalimumab concentration in the sample. The Adalimumab concentration in the sample is calculated based on the standard curve.

Application

This kit is designed for the quantitative detection of adalimumab residues in meat tissue, serum, and urine samples.

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