ELK011 – MEBEP Bio Science https://www.mebep.com make experiment be predigest Thu, 11 Dec 2025 03:25:22 +0000 en-US hourly 1 https://wordpress.org/?v=6.9.4 Human Anti-adalimumab antibodies ELISA Kit https://www.mebep.com/elisa-kit/human-anti-adalimumab-antibodies-elisa-kit/ Thu, 11 Dec 2025 03:25:21 +0000 https://mebep.com/?p=3527 Manual

Product Number: ELK011

Shipping and Storage

  1. Reagent kit storage: 2-8℃.
  2. Validity period: 6 months.

Component

Component96T
30× Concentrated Wash Buffer20mL×1
Enzyme Conjugate6mL×1
Microplate (Coated)12 wells×8 strips
Sample Diluent6mL×1
Chromogen Substrate A6mL×1
Chromogen Substrate B6mL×1
Stop solution6mL×1
Positive control0.5mL×1
Negative control0.5mL×1

Description

This kit uses the double antigen sandwich method to determine the level of human anti Adalimumab Ab in specimens. Coat the microporous plate with purified antigen to prepare solid-phase antigen. Add anti Adalimumab Ab antibodies to the coated micropores in sequence, and then bind with HRP labeled antigen to form an antigen antibody enzyme labeled antigen complex. After thorough washing, add substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and to the final yellow under the action of acid. The depth of color is positively correlated with the presence of Adalimumab Ab antibodies in the sample. Measure the absorbance (OD value) at a wavelength of 450nm using an enzyme-linked immunosorbent assay (ELISA) reader, and calculate the concentration of human anti adalimumab antibody (Adalimumab Ab) in the sample using a standard curve.

Application

This kit is used to determine the content of anti Adalimumab Ab antibodies in human serum, plasma, and related liquid samples.

Testing Scope

3ng/L -180ng/L

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