ELK012 – MEBEP Bio Science https://www.mebep.com make experiment be predigest Thu, 11 Dec 2025 03:26:30 +0000 en-US hourly 1 https://wordpress.org/?v=6.9.4 Avian AP33 (Adhesion Protein 33 kDa) ELISA Kit https://www.mebep.com/elisa-kit/avian-ap33-adhesion-protein-33-kda-elisa-kit/ Thu, 11 Dec 2025 03:26:28 +0000 https://mebep.com/?p=3529 Manual

Product Number: ELK012

Shipping and Storage

  1. Reagent kit storage: 2-8℃.
  2. Validity period: 6 months.

Component

Component96T
30× Concentrated Wash Buffer20mL×1
Enzyme Conjugate6mL×1
Microplate (Coated)12 wells×8 strips
Sample Diluent6mL×1
Chromogen Substrate A6mL×1
Chromogen Substrate B6mL×1
Stop solution6mL×1
Positive control0.5mL×1
Negative control0.5mL×1

Description

This kit uses the double antibody sandwich method to determine the level of avian AP33 protein (AP33) in specimens. Coat a microplate with purified avian AP33 protein (AP33) antibody to prepare a solid-phase antibody. Add avian AP33 protein (AP33) to the coated micropores in sequence, and then bind with HRP labeled avian AP33 protein (AP33) antibody to form an antibody antigen enzyme labeled antibody complex. After thorough washing, add substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and to the final yellow under the action of acid. The depth of color is positively correlated with the presence of avian AP33 protein (AP33) in the sample. Measure the absorbance (OD value) at a wavelength of 450nm using an enzyme-linked immunosorbent assay (ELISA) reader, and calculate the concentration of avian AP33 protein (AP33) in the sample using a standard curve.

Application

This kit is used to determine the content of avian AP33 protein (AP33) in poultry serum, plasma, and related liquid samples.

Testing Scope

5ng/L -260ng/L

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