ELK019 – MEBEP Bio Science https://www.mebep.com make experiment be predigest Thu, 11 Dec 2025 03:36:23 +0000 en-US hourly 1 https://wordpress.org/?v=6.9.4 Human ACCPA ELISA Kit https://www.mebep.com/elisa-kit/human-accpa-elisa-kit/ Thu, 11 Dec 2025 03:36:21 +0000 https://mebep.com/?p=3545 Manual

Product Number: ELK019

Shipping and Storage

  1. Reagent kit storage: 2-8℃.
  2. Validity period: 6 months.

Component

Component96T
30× concentrated wash buffer20mL×1
Enzyme conjugate6mL×1
Microplate (Coated)12 wells×8 strips
Sample diluent6mL×1
Chromogen substrate A6mL×1
Chromogen substrate B6mL×1
Stop solution6mL×1
Standard (640ng/mL)0.5mL×1
Standard diluent1.5mL×1

Description

This kit uses the double antigen sandwich method to determine the level of human anti cyclic citrullinated peptide antibody (ACCPA) in specimens. Coat the microporous plate with purified antigen to prepare solid-phase antigen. Add anti cyclic citrullinated peptide antibody (ACCPA) to the coated micropores in sequence, and then bind with HRP labeled antibody to form an antigen antibody enzyme labeled antigen complex. After thorough washing, add substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and to the final yellow under the action of acid. The depth of color is positively correlated with the presence of anti cyclic citrullinated peptide antibodies (ACCPA) in the sample. Measure the absorbance (OD value) at a wavelength of 450nm using an enzyme-linked immunosorbent assay (ELISA) reader, and calculate the concentration of human anti cyclic citrullinated peptide antibody (ACCPA) in the sample using a standard curve.

Application

This kit is used to determine the content of anti cyclic citrullinated peptide antibodies (ACCPA) in human serum, plasma, and related liquid samples.

Testing Scope

10ng/L – 320ng/L

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