ELK020 – MEBEP Bio Science https://www.mebep.com make experiment be predigest Thu, 11 Dec 2025 03:37:29 +0000 en-US hourly 1 https://wordpress.org/?v=6.9.4 Human BTLA ELISA Kit https://www.mebep.com/elisa-kit/human-btla-elisa-kit/ Thu, 11 Dec 2025 03:37:27 +0000 https://mebep.com/?p=3547 Manual

Product Number: ELK020

Shipping and Storage

  1. Reagent kit storage: 2-8℃.
  2. Validity period: 6 months.

Component

Component96T
30× concentrated wash buffer20mL×1
Enzyme conjugate6mL×1
Microplate (Coated)12 wells×8 strips
Sample diluent6mL×1
Chromogen substrate A6mL×1
Chromogen substrate B6mL×1
Stop solution6mL×1
Standard (240ng/mL)0.5mL×1
Standard diluent1.5mL×1

Description

This kit uses the double antibody sandwich method to determine the level of human B/T lymphocyte attenuation factor (BTLA) in the specimen. Coat a microplate with purified human B/T lymphocyte attenuation factor (BTLA) antibody to prepare a solid-phase antibody. Add B/T lymphocyte attenuation factor (BTLA) to the coated micropores in sequence, and then bind with HRP labeled B/T lymphocyte attenuation factor (BTLA) antibody to form an antibody antigen enzyme labeled antibody complex. After thorough washing, add substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and to the final yellow under the action of acid. The depth of color is positively correlated with the B/T lymphocyte attenuation factor (BTLA) in the sample. Measure the absorbance (OD value) at a wavelength of 450nm using an enzyme-linked immunosorbent assay (ELISA) reader, and calculate the concentration of human B/T lymphocyte attenuation factor (BTLA) in the sample using a standard curve.

Application

This kit is used to determine the content of B/T lymphocyte attenuation factor (BTLA) in human serum, plasma, and related liquid samples.

Testing Scope

3ng/L -150ng/L

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