ELK026 – MEBEP Bio Science https://www.mebep.com make experiment be predigest Thu, 11 Dec 2025 03:44:48 +0000 en-US hourly 1 https://wordpress.org/?v=6.9.4 Human RBP4 ELISA Kit https://www.mebep.com/elisa-kit/human-rbp4-elisa-kit/ Thu, 11 Dec 2025 03:44:46 +0000 https://mebep.com/?p=3559 Manual

Product Number: ELK026

Shipping and Storage

  1. Reagent kit storage: 2-8℃.
  2. Validity period: 6 months.

Component

Component96T
30× concentrated wash buffer20mL×1
Enzyme conjugate6mL×1
Microplate (Coated)12 wells×8 strips
Sample diluent6mL×1
Chromogen substrate A6mL×1
Chromogen substrate B6mL×1
Stop solution6mL×1
Standard (80μg/L)0.5mL×1
Standard diluent1.5mL×1

Description

This kit uses the double antibody sandwich method to determine the level of human retinol binding protein 4 (RBP-4) in specimens. Coat a microplate with purified human retinol binding protein 4 (RBP-4) antibody to prepare a solid-phase antibody. Add retinol binding protein 4 (RBP-4) to the coated micropores in sequence, and then bind with HRP labeled retinol binding protein 4 (RBP-4) antibody to form an antibody antigen enzyme labeled antibody complex. After thorough washing, add substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and to the final yellow under the action of acid. The depth of color is positively correlated with retinol binding protein 4 (RBP-4) in the sample. Measure the absorbance (OD value) at a wavelength of 450nm using an enzyme-linked immunosorbent assay (ELISA) reader, and calculate the concentration of human retinol binding protein 4 (RBP-4) in the sample using a standard curve.

Application

This kit is used to determine the content of retinol binding protein 4 (RBP-4) in human serum, plasma, and related liquid samples.

Testing Scope

1μg/L -40μg/L

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