HQ060212001L – MEBEP Bio Science https://www.mebep.com make experiment be predigest Tue, 31 Dec 2024 01:12:42 +0000 en-US hourly 1 https://wordpress.org/?v=6.9.4 Ni Sepharose High Performance https://www.mebep.com/nucleic-acid-purification-raw-materials-consumables/ni-sepharose-high-performance/ Wed, 27 Nov 2024 09:28:45 +0000 https://mebep.com/?p=1955 Manual

Product Number: HQ060212001L

Shipping and Storage

4-30℃ in 20% ethanol

Description

Ni Sepharose High Performance is capable of separation and purification by the interactionbetween Ni2+ and certain amino acids (mainly histidine, cysteine, and tryptophan) on theprotein side chain, and is applicable for the separation and purification of His-tag proteinsand biomolecules that interact with Ni2+.

MatrixHighly cross-linked 6% sepharose
Range of particle size25-45µm
Mean particle size35±5µm
Binding capacity≥ 40 mg (His-tag protein)/mL (media)
pH stability*3 - 12 (long-term) 2 - 14 (short-term)
Chemical stability**All common aqueous solutions and buffers; Avoid chelating agents (such as EDTA and EGTA) and reducing agents (such as DTT and DTE)
Flow rate≥150cm/h

*: Stability refers to the stability of the media before chelating with metal ions.

Buffer0.05M sodium phosphate, pH 7.4 0.1M Tris-HCl, pH 7.4 0.1M Tris-acetate, pH 7.4 0.1M HEPES, pH 7.4 0.1M MOPS, pH 7.4 0.1M sodium acetate, pH 4
Denaturant8M Urea 6M Gua-HCl
Detergent2% Triton X-100 2% Tween 20 2% NP-40 2% Cholate 1% CHAPS
Reducing agent*0.005M DTE 0.005M DTT 0.02M β-mercaptoethanol 0.005M TCEP 0.01M reduced glutathione
Other additives0.5M Imidazole 20% Ethanol 50% Glycerol 0.1M Na2SO4 1.5M NaCl 0.001M EDTA** 0.06M Citrate

Package

 Product NamePackageProduct Number
Ni Sepharose High Performance25mlHQ060212025M
Ni Sepharose High Performance100mlHQ060212100M
Ni Sepharose High Performance500mlHQ060212500M
Ni Sepharose High Performance1LHQ060212001L
Ni Sepharose High Performance5LHQ060212005L
Ni Sepharose High Performance20LHQ060212020L

Features

  1. Fast and simple operation (one-step purification).
  2. It has wide scope of application, with simple operation, and is applicable for gravity column and pre-packed column (peristaltic pump or chromatography system).
  3. Multiple options are available: it can be chelated with various metal ions (such as Cu2+, Zn2+, Fe2+, Co2+, Ca2+, etc.) for use.
  4. Compared with Ni Focurose FF (IDA), it has advantages of low Ni2+ dropout and wide reagent compatibility.
  5. Small particles, high resolution.

Note: Avoid using phosphate buffers (since there may be precipitates) when chelating with Ca2+.

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