Product Number: RNK4501LY

Shipping and Storage

Freeze dried powder is stored at 4 ℃, and after reconstitution, the solution is stored at -20 ℃

Components

Description

DNase I is a deoxyribonuclease that requires a divalent cation and can be used to degrade single stranded or double stranded DNA. The principle is that DNase I hydrolyzes phosphate diester bonds to produce single nucleotides or oligonucleotides with 5 '- phosphate groups and 3' - OH groups. Both Mg²⁺ and Mn²⁺ can activate the activity of DNase I, while Ca²⁺ concentration directly affects enzyme activity. When Mg²⁺ is present, it can randomly generate incisions on each single strand of double stranded DNA; In the presence of Mn²⁺, double stranded DNA can be broken and fragmented. Used for the preparation of RNA without DNA contamination, reverse transcription, and in vitro transcription experiments.

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