S080203 – MEBEP Bio Science https://www.mebep.com make experiment be predigest Fri, 16 Jan 2026 02:13:37 +0000 en-US hourly 1 https://wordpress.org/?v=6.9.4 Ni-NTA Agrose 6 Fast Flow https://www.mebep.com/nucleic-acid-purification-raw-materials-consumables/ni-nta-agrose-6-fast-flow/ Fri, 24 Oct 2025 10:00:09 +0000 https://mebep.com/?p=3363 Manual

Product Number: S080203

Shipping and Storage

  1. Packaging: PP material reagent bottle sealed packaging, with a specification of 25mL. Please consult for other packaging.
  2. Storage: 2-8℃, 20% ethanol.
  3. Shelf life: 24 months.

Component

ComponentS080203
Ni-NTA Agrose 6 Fast Flow25mL

Description

Ni NTA SweAgorase 6 Fast Flow is a metal ion chelating affinity chromatography separation medium formed by binding NTA ligands chelated with nickel ions onto agarose microspheres. Fixed metal ion affinity chromatography (NTA) utilizes the interaction between Ni2+and the side chains of certain amino acids (mainly histidine, with small amounts of cysteine and tryptophan) on proteins for separation and purification.

Features: Wide range of use, simple operation, suitable for gravity columns and pre installed columns;

High flow rate, high loading capacity, low Ni2+detachment, and wide compatibility of reagents;

Multiple selection can chelate various metal ions for use, usually Zn2+, Ni2+, Cu2+or Co2+.

Performance

IndicatorDescription
Product Content66% (v/v) agarose gel, stored in 20% ethanol
Gel type6% cross-linked agarose gel
Ligands-NTA-Ni2+
Particle size range (μm)45 - 165
Average particle size (μm)90
Combined with load capacity20-50 mg (His tagged protein)/mL medium
Ligand density (μmol/mL)~ 25
Operating flow rate (cm/h)≤600
Pressure resistance (MPa)0.3
Chemical stability rangeCommonly used aqueous buffer solutions; 0.01 M hydrochloric acid, 0.1 M sodium hydroxide (1 week); 1 M sodium hydroxide, 70% acetic acid (1 day); 2% SDS (1 hour); 30% 2-propanol (30 minutes)

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