Description

CSM Taq DNA Polymerase (Cold sensitive mutant Taq DNA Polymerase ) is a kind of hot start DNA Polymerase. Source from the mutant Ecoli. CSM Taq DNA Polymerase unlike the monoclonal antibody-based hot start Taq or chemical modified hot start Taq, Cold Taq is the cold-sensitive mutant Taq. It retains the hot start ability throughout the whole amplification cycle.

Cold sensitive mutant Taq DNA Polymerase is designed for Hot Start PCR, it is not at low temperature. It offers excellent specificity and two-fold higher fidelity than wild-type Taq. It is designed for PCR with difficult templates such as GC-rich fragments and microsatellites. Cold sensitive mutant Taq DNA Polymerase is particularly well suited to primer extension of Single Nucleotide Polymorphism (SNP) markers.

Cold sensitive mutant Taq DNA Polymerase maintains excellent specificity and minimal background even in conditions designed for high yield. Even on genomic templates, the enzyme can be used with MgCl2+concentrations as high as 10 mM.

Cold sensitive mutant Taq DNA Polymerase is capable of extending through difficult regions, e.g. regions, which include inverted tandem repeats and those with high amounts of secondary structure.

Cold sensitive mutant Taq DNA Polymerase work in a unique way, involving improved nucleotide selection at the active site, and a much lower rate of mismatch extension, meaning that only perfectly aligned primers will be extended. As a result, the enzyme can give even higher specificity than hot start (manual or automatic) techniques without the need for inconvenient pre-incubation steps.

Conc. 5 U/μl

Package: Bulk

Store: at -20°C

Applications

1 Hot start PCR amplification

2 Specific amplification of complex cDNA and genomic template, for amplification of difficult templates, such as GC-rich fragments and microsatellites

3 Primer extension of SNP markers

4 Amplification of genomic DNA targets up to 10 kb with high fidelity, specificity, and sensitivity

5 Amplification from low copy number DNA template, high through-put Hot Start PCR with high specificity, sensitivity, and yield

6 Routine diagnostic Hot Start PCR requiring high reproducibility.

7 Real-Time PCR

8 Multiple PCR

9 Generation of PCR products for TA cloning

Quality Control

Functional absence of double and single-stranded endonuclease activity;

Purity>99% test by SDS gel electrophoresis;

Each lot of CSM Taq DNA Polymerase is assayed for amplification from as little as 10 ng of human genomic DNA; Retain full activity at room temperature for one week; No host DNA residue.

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The mTaq DNA Polymerase is the mutant of the general Taq DNA Polymerase. mTaq has the better anti inhibitor capacity and high amplification efficiency can directly use for amplification of blood interstitial fluid and other crude templates. The extending speed is 1-2 kb/min. The PCR product with an “A” on 3′end, can be cloned in TA vector. 

Concentration: 5 u/µl

Package: Bulk

Storage: Store at -20℃ 

Features

• Can directly use for amplification of frozen or fresh blood, template does not need pretreatment or purification.
• Directly amplify the template of dried blood and interstitial fluid.
• Amplify the DNA from the crude template such as feces and soil extractive.
• The amplification speed is twice of the general Taq DNA Polymerase.

Unit Definition

One unit of mTaq DNA Polymerase High Fidelity incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C. 

Quality Control

Functional absence of double and single-stranded endonuclease activity; 

Purity>99% test by SDS gel electrophoresis;

Each lot of mTaq DNA Polymerase is assayed for amplification from as little as 10 ng of human genomic DNA; Retain full activity at room temperature for one week; No host DNA residue. 

Application

• Crude template DNA amplification.
• Genotyping
• complex DNA template amplification.

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Concentration: 5u/µl

Storage: Store at –20°C

Description

Taq Plus DNA Polymerase is a special formulation designed for amplifying large fragments. The main component is Taq DNA Polymerase, and Pfu DNA Polymerase are added to enhance the efficiency of the amplification reaction. Theoretically, Taq Plus produces significantly higher yields of PCR products than ordinary Taq Polymerase, especially for fragments >1kb, and can amplify up to 20kb. Taq Plus also contains a proofreading activity that reduces the error rate of Taq Polymerase. Most of the amplified DNA fragments have a 3´A overhanging. However, a small percentage of the amplified DNA fragments are blunt-ended. Taq Plus is suitable as a direct replacement for ordinary Taq Polymerase in most applications.

Features

High fidelity: with an error frequency of 1.6X10-6 during DNA synthesis.

Higher yield: Taq Plus increases the efficiency of the polymerization reaction, resulting in a great percentage of extenuation reaction completion up to 20kb.

Unit Definition

One unit of the enzyme catalyzes the incorporation of 10nmole of deoxyribonucleotides into a polynucleotide fraction in 30min at 74℃.

Storage Buffer

20mM Tris-HCl, pH 8.0, 100mM KCl, 0.1mM EDTA, 5mM DTT, 50% glycerol, 0.5% NP40 and 0.5% Tween 20.

10X Reaction Buffer (Mg2+ Plus)

500mM KCl, 100mM Tris-HCl (pH 9.0 at 25℃) and 1% Triton X-100, 100mM (NH4)2SO4, 15mM MgCl2, PCR enhancer

Applications

Long PCR (up to 20 kb), PCR cloning, RT-PCR etc.

Quality Control

No contaminating endonuclease or exonuclease activity was detected. Functionally tested in PCR.

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Features

• Long PCR products
–– up to 40 kb with viral DNA as a template
–– up to15 kb with genomic DNA as a template
• Ideal for GC-rich templates up to 85% GC.
• Fidelity is three times higher than with Taq DNA Polymerase.
• High yields.
• Incorporates modified nucleotides.

Concentration

5 u/μl

Quality Control

Functionally tested in the generation of 40 kb amplicon with lambda DNA as a template.

Storage Buffer

The enzyme is supplied in: 20mM Tris-HCl (pH 8.0); 0.1mM EDTA; 1mM DTT; 100mM KCl; Stabilizers; 50% glycerol.

Storage

Store at -20°C.

Reaction PCR Mixture Set Up and Recommended thermal cycling conditions

The flowing is only an example that takes a 20KB human genomic DNA as the template, that only for reference, It can be adjusted according to the length and the sequence of the template and the primer.

Component Volume Final Concentration
Template DNA <1 ug as required
Forward Primer (10 μM) 1 μl 0.2-0.4 μM each
Reverse Primer (10 μM) 1 μl 0.2-0.4 μM each
10×LA Taq Buffer 5 μl 1×
2.5 mM dNTPs 4 μl 0.2 mM
LA Taq DNA polymerase 0.5μl 2.5 unit
ddH2O to final volume 50μl Not applicable
94℃ 3 min
94℃ 30 sec
55℃ 30 sec 30 cycles
72℃ 1 min
72℃ 5 min

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Taq DNA polymerase is isolated from the E. coli cloning Thermus aquaticus. The molecular weight of the product is approximately 94 kDa. EasyTaq DNA polymerase has the 5′to 3′DNA polymerase activity and 5′to 3′exonuclease activity without 3′-5′exonuclease activity. The extending speed is 1-2 kb/min. There is an "A" on the 3′end. The PCR product can be cloned in TA vector.

Conc. 5 U/μl

Store at -20°C

Characteristics

1. high-sensitivity

2. high amplification efficiency

Unit Definition

One unit of Platinum Taq DNA Polymerase High Fidelity incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.

Quality Control

This product has passed the following quality control assays: functional absence of double- and single-stranded endonuclease activity; >90% homogeneous by SDS gel electrophoresis. Each lot of EasyTaq DNA Polymerase is assayed for amplification from as little as 10 ng of human genomic DNA.

Storage Buffer

20 mM Tris-HCl (pH 8.0),0.1 mM EDTA,1 mM DTT,100 mM KCl,Stabilizers,50% glycerol.

10X PCR reaction Buffer

500 mM KCl,100 mM Tris-Cl (pH8.5 at 25℃),1% Triton X-100,15 mM MgCl2

Reaction Mixture Set Up

Component Volume Final Concentration

Template DNA <0.5 ug as required

Forward Primer (10 μM) 1 μl 0.2-0.4 μM each

Reverse Primer (10 μM) 1 μl 0.2-0.4 μM each

10×PCR reaction Buffer 5 μl 1×

2.5 mM dNTPs 4 μl 0.2 mM

Taq DNA polymerase 0.5μl 2.5 unit

ddH2O to final volume 50μl Not applicable

Recommended thermal cycling conditions

94℃ 2-5 min

94℃ 30 sec

50-60℃ 30 sec 30-35 cycles

72℃ 1-2 kb/min

72℃ 5-10 min

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Description

The 2×TaqMan Master Mix is a special mixture for Probe real time PCR(TaqMan, Molecular Beacon etc.). Which include the HotStar DNA Polymerase, PCR Buffer, MgCl2 , dNTPs, ROX and the stabilizing agent. The HotStar Taq DNA polymerase which in the mix is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer dimers and providing higher specificity of DNA amplification. The functional activity of the enzyme is activated in 10 minute incubation at 95°C. The unique reaction buffer with the special Hotstar enzyme highly increased the Amplification efficiency. The fluorescence signals is stronger, the sensitivity is higher can detect the Single copy sequences. The Rox in the mix can be used for the correction of the fluorescent signal in the qPCR. The 2×TaqMad qPCR master Mix produces a optimal results in qPCR.

Note

1. Before use, please upside down gently for blending, avoid foam, and short centrifuge.
2. The Master Mix contain the ROX need to avoid the hard light when use and storage.
3. Avoid repeated freezing and thawing, frequently use can be stored at2-8℃, for long term storage can be store at -20℃.

Store at -20℃

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Description of the 2x PCR master mix

This PCR master mix contains the Taq DNA Polymerase, dNTPs, and optimized buffer but without any dye in it. This Taq Mastermix is provided at 2× concentration and used at 1× concentration by adding the template, primer, and H2O. It has the 5′to 3′DNA polymerase activity and 5′to 3′exonuclease activity without 3′-5′exonuclease activity. The extending speed is 1-2 kb/min. There is an "A" on the 3′end. The PCR product can be cloned in a TA vector.

Quality Control

This product has passed the following quality control assays: functional absence of double- and single-stranded endonuclease activity; >90% homogeneous by SDS gel electrophoresis. Each lot of our Taq DNA Polymerase is assayed for amplification from as little as 10 ng of human genomic DNA 

Application 

• PCR
• Primer Extension
• Microarray Analysis
• High-Throughput PCR

Storage: Store at -20°C

Shipping with blue ice

Reaction Mixture Set Up

Template <0.5 μg

Forward Primer (10 μM) 1 μl

Reverse Primer (10 μM) 1 μl

2×TransTaq PCR SuperMix 25 μl

ddH2O to final volume 50 μl

No repeated freezing and thawing and split charging for good amplification.

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This Taq PCR Master mix contains Taq DNA Polymerase, dNTPs, and optimized buffer, and blue dye. This Taq Mastermix is provided at 2× concentration and used at 1× concentration by adding the template, primer, and H2O. It has the 5′to 3′DNA polymerase activity and 5′to 3′exonuclease activity without 3′-5′exonuclease activity. The extending speed is 1-2 kb/min. There is an “A” on the 3′end. The PCR product can be cloned in a TA vector.

Quality Control

This product has passed the following quality control assays: functional absence of double- and single-stranded endonuclease activity; >90% homogeneous by SDS gel electrophoresis. Each lot of Taq DNA Polymerase is assayed for amplification from as little as 10 ng of human genomic DNA.

Application 

• PCR
• Primer Extension
• Microarray Analysis
• High-Throughput PCR

Storage

Store at -20°C

Notes

no repeated freezing and thawing and split charging for good amplification.

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It contains Taq DNA Polymerase, dNTPs, and an optimized buffer. This Taq Mastermix is provided at 2× concentration and used at 1× concentration by adding the template, primer, and H2O.

Application:

PCR Primer, Extension, Microarray Analysis, High-Throughput PCR

Storage: Store at -20°C

Reaction Mixture Set Up

Template <0.5 μg

Forward Primer (10 μM) 1 μl

Reverse Primer (10 μM) 1 μl

2×TransTaq PCR SuperMix 25 μl

ddH2O to final volume 50 μl 

Recommended thermal cycling conditions

94℃ 2-5 min

94℃ 30 sec

50-60℃ 30 sec 30-35 cycles

72℃ 1 min/1-2 kb

72℃ 5-10 min

 Notes: no repeated freezing and thawing and split charging for good amplification.

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The HotStar Taq Mastermix includes the HotStar DNA Polymerase, PCR Buffer, Mg2+, dNTPs, the blue dye, and the stabilizing agent. The HotStar Taq DNA polymerase has been chemically modified by the addition of heat-labile blocking groups to its amino acid residues. The enzyme is inactive at room temperature, avoiding the extension of non-specifically annealed primers or primer dimers and providing higher specificity of DNA amplification. The functional activity of the enzyme is activated in 10-minute incubation at 95°C. The activated enzyme maintains the same functionality as Taq DNA polymerase: Has the 5′to 3′DNA polymerase activity and 5′to 3′exonuclease activity without 3′-5′exonuclease activity. The extending speed is 1 kb/30 sec. There is an "A" on the 3′end. The PCR product can be cloned in a TA vector.

Applications

Hot start PCR amplification Specific amplification of complex cDNA and genomic template Amplification from low copy number DNA template Real-Time PCR Multiplex PCR Generation of PCR products for TA cloning

Quality Control

Functional absence of double and single-stranded endonuclease activity; Assayed for amplification from as little as 10 ng of human genomic DNA; Retain full activity at room temperature for one week; No host DNA residue.

Storage

Store at -20°C

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