TSC01 – MEBEP Bio Science https://www.mebep.com make experiment be predigest Tue, 20 Jan 2026 07:57:24 +0000 en-US hourly 1 https://wordpress.org/?v=6.9.4 TSC Agar https://www.mebep.com/culture-medium/tsc-agar/ Tue, 20 Jan 2026 07:55:33 +0000 https://www.mebep.com/?p=3767 Manual

Product Number: TSC01

Product details

Formula of culture medium (per liter); pH7.6 ± 0.2(25℃)

ComponentWeight
Tryptone25.0g
Enzymatic digest of soybean meal5.0g
Yeast extract powder5.0g
Sodium Pyrosulfite1.0g
Ferric Ammonium Citrate1.0g
Agar15.0g

Instructions

Weigh 42.0g of this product into 1L of distilled or deionized water, heat and boil until completely dissolved, divide 250mL per bottle, sterilize at 121℃ for 15 minutes, shake well after sterilization to prevent agar from settling at the bottom of the vessel and solidifying, cool to around 50℃, add 0.1g D-cycloserine per 250mL of culture medium, gently shake well, and pour onto a plate.

If necessary, 25mL of 50% yolk saline suspension can be selectively added to every 250mL of culture medium.

Application

Used for Plate Counting of Clostridium perfringens.

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