Vaccinia Capping enzyme is a recombinant protein from Vaccinia virus. Vaccinia Capping Enzyme can add 7-methylguanylate cap structure (Cap 0) to the 5′ end of RNA. In eukaryotes, these terminal cap structures are involved in stabilization, transport, translation of mRNAs. Vaccinia Capping enzyme is composed of two subunits (D1R, D12L), and has three enzymatic activities (RNA triphosphatase, guanylyltransferase by the D1R subunit and guanine methyltransferase by the D12L subunit). All necessary for addition of a complete Cap 0 structure, m7Gppp5’N). In vitro, transcripts can be capped in the presence of reaction buffer, GTP, and the methyl donor (SAM).

Source

Recombinant E.coli

Concentration and Size

10U/μL

Unit Definition

One unit is defined as the amount of enzyme required to incorporate 10pmol of GTP into an 80 nt transcript in 1 hour at 37℃.

Storage Buffer

20mM Tris-HCl (pH 8.0), 0.1mM EDTA, 100mM NaCl, 1mM DTT, 0.1%(v/v) Triton X-100, 50%(v/v) glycerol.

Storage Conditions

-20 °C

Quality Assurance

Free of Endonuclease, Exonuclease, and RNase activities.

Physical Purity

Purity >95% by SDS-PAGE.

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