Product Number: MARG01
Shipping and Storage
Component
Component | 96T | 48T |
Microporous enzyme-linked immunosorbent assay plate | 12 holes×8 strips | 12 holes×4 strips |
Negative control | 0.5mL | 0.5mL |
Positive control | 0.5mL | 0.5mL |
Detect antibody HRP | 10mL | 5mL |
20× washing buffer solution | 25mL | 15mL |
Substrate A | 6mL | 3mL |
Substrate B | 6mL | 3mL |
Stop solution | 6mL | 3mL |
Microplate Sealers | 2 sheets | 2 sheets |
Description
The reagent kit adopts indirect enzyme-linked immunosorbent assay (ELISA). Add the sample and HRP labeled detection antibody to the pre coated micro well with mouse anti rabies virus IgG antigen, incubate and thoroughly wash. Using substrate TMB for color development, TMB is converted to blue under the catalysis of peroxidase and to the final yellow under the action of acid. Measure the absorbance (OD value) at a wavelength of 450nm using an enzyme-linked immunosorbent assay (ELISA) reader, and compare it with the CUTOFF value to determine the presence or absence of anti RV IgG antibodies in the sample.