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2025-12-09BigDye3.1 sequecing kit
Product Number: SGS48
BDT 3.1 Sanger Sequencing Kit
Shipping and Storage
- 5×Sequencing Buffer store at 2-8℃, BigDye3.1 and Sanger Sequency Mix store at -20℃.
- Correctly stored, with a validity period of 12 months from receipt.
Components
| Components | Specifications | Reaction count (5µL) |
| 5×Sequencing Buffer | 1mL | About 1000 |
| BigDye3.1 | 1mL | Approximately 3000-4000 PCR products or 2000-2500 plasmid sequencing reactions |
| Sanger Sequencing Mix | 1mL | About 500 PCR sequences or 350 plasmid sequences |
Description
- 5×Sequency Buffer and BigDye3.1 are both original products sold after packaging.
- Sanger Sequency Mix is an optimized combination of 5 x Sequency Buffer and BigDye3.1, containing a certain amount of auxiliary additives.
- In theory, 1ml BigDye3.1 needs to be paired with 2-3mL 5 x Sequency Buffer.
- Purpose and scope of application: First generation sequencing reaction.
Note
- The actual cost of the unit is relatively high, be careful when adding samples to reduce sample loss.
- Before each experiment, perform instantaneous centrifugation to further reduce the loss of the tube wall.
