Aflatoxin B2 (AFB2) Enzyme Linked Immunoassay (ELISA) Kit
2025-12-31Aflatoxin G2 (AFG2) Enzyme Linked Immunoassay (ELISA) Kit
2025-12-31Aflatoxin G1 (AFG1) Enzyme Linked Immunoassay (ELISA) Kit
Product Number: ELK030
Shipping and Storage
- The reagent kit should be stored at 2-8℃ and should not be frozen.
- Unused microplates should be sealed and stored dry.
Component
| Component | 96T |
| Detachable enzyme-linked immunosorbent assay (ELISA) plates for pre coated aflatoxin G1 (AFG1) conjugated antigen | 1 plate(12 holes×8 strips) |
| Aflatoxin G1 (AFG1) standard | 6 bottles (1mL/bottle) |
| Anti aflatoxin G1 (AFG1) antibody enzyme conjugate | 6mL |
| Chromogen solution A | 6mL |
| Chromogen solution B | 6mL |
| Stop solution | 6mL |
| Sample diluent | (10×, 6mL) |
| Concentrated wash buffer | (20×, 6mL) |
Description
This kit uses a competitive ELISA method, in which aflatoxin G1 (AFG1) conjugated antigen is coated on a microplate, and aflatoxin G1 (AFG1) standard or sample is added. Free aflatoxin G1 (AFG1) competes with the pre coated aflatoxin G1 (AFG1) conjugated antigen on the microplate for anti aflatoxin G1 (AFG1) antibody enzyme labeling. TMB substrate is used for color development, and the color changes from blue to yellow after adding termination solution. The absorbance value is inversely proportional to the content of aflatoxin G1 (AFG1) in the sample, and the content of aflatoxin G1 (AFG1) in the sample is calculated by the standard curve.
Application
This kit is used for quantitative detection of aflatoxin G1 (AFG1) residues in corn, feed, fish, shrimp, and meat tissues (such as chicken, beef, and pork), eggs, honey, milk, serum, and urine samples.
Materials required but not supplied
- Equipment: 450nm wavelength enzyme-linked immunosorbent assay (ELISA) reader, pulverizer, graduated cylinder, oscillator, funnel, Whatman No 1 or equivalent filter paper, micropipette.
- Reagents: Deionized water or distilled water, Methanol.
Note
- Please read the instructions carefully before using the kit.
- Do not use expired kits.
- Before use, bring all reagents to room temperature (25±2°C). It is recommended to equilibrate for at least 2 hours.
- The standard contains aflatoxin G1 (AFG1), so special attention should be paid when using it and gloves should be worn during operation.
- The stop solution contains sulfuric acid. Prevent skin burns and corrosion of clothing.
- Do not reuse pipette tips for different standards or samples, as this may affect the test results.
- Do not mix reagents from different kit lots; do not reuse pipette tips for different standards or samples, as this may affect the test results.
- The sample diluent provided in this kit must be used for diluting samples; otherwise, results may be affected.
- Avoid generating bubbles when mixing reagents.
