Aflatoxin G2 (AFG2) Enzyme Linked Immunoassay (ELISA) Kit
2025-12-31Zea zearalenone (ZEN) enzyme-linked immunosorbent assay (ELISA) kit
2025-12-31Ochratoxin A (OTA) Enzyme Linked Immunoassay (ELISA) Kit
Product Number: ELK032
Shipping and Storage
- The reagent kit should be stored at 2-8℃ and should not be frozen.
- Unused microplates should be sealed and stored dry.
Component
| Component | 96T |
| Detachable enzyme-linked immunosorbent assay (ELISA) plates for pre coated ochratoxin A (OTA) conjugated antigens | 1 plate(12 holes×8 strips) |
| Ochratoxin A (OTA) standard | 6 bottles (1mL/bottle) |
| Anti ochratoxin A (OTA) antibody enzyme conjugate | 6mL |
| Chromogen solution A | 6mL |
| Chromogen solution B | 6mL |
| Stop solution | 6mL |
| Sample diluent | (10×, 6mL) |
| Concentrated wash buffer | (20×, 6mL) |
Description
This kit uses a competitive ELISA method to coat a microplate with ochratoxin A (OTA) conjugated antigen, add ochratoxin A (OTA) standard or sample, and free ochratoxin A (OTA) competes with the pre coated ochratoxin A (OTA) conjugated antigen on the microplate for anti ochratoxin A (OTA) antibody enzyme label. The TMB substrate is used for color development, and the color changes from blue to yellow after adding termination solution. The absorbance value is inversely proportional to the content of ochratoxin A (OTA) in the sample, and the content of ochratoxin A (OTA) in the sample is calculated by the standard curve.
Application
This kit is used for quantitative detection of residual aflatoxin A (OTA) in feed, fish, shrimp, and meat tissues (such as chicken, beef, and pork), food, eggs, honey, milk, serum, and urine samples.
Materials required but not supplied
- Equipment: 450nm wavelength enzyme-linked immunosorbent assay (ELISA) reader, pulverizer, graduated cylinder, oscillator, funnel, Whatman No 1 or equivalent filter paper, micropipette.
- Reagents: Deionized water or distilled water, Methanol.
Note
- Please read the instructions carefully before using the kit.
- Do not use expired kits.
- Before use, bring all reagents to room temperature (25±2°C). It is recommended to equilibrate for at least 2 hours.
- The standard contains ochratoxin A (OTA), so special attention should be paid when using it and gloves should be worn during operation.
- The stop solution contains sulfuric acid. Prevent skin burns and corrosion of clothing.
- Do not reuse pipette tips for different standards or samples, as this may affect the test results.
- Do not mix reagents from different kit lots; do not reuse pipette tips for different standards or samples, as this may affect the test results.
- The sample diluent provided in this kit must be used for diluting samples; otherwise, results may be affected.
- Avoid generating bubbles when mixing reagents.
