Manual

Kit(Qualitative)

Product Number: ELK10556

Shipping and Storage

1. Reagent kit storage: 2-8℃.
2. Validity period: 6 months.

Component

Component	96T
30× concentrated wash buffer	20mL×1
Enzyme conjugate	6mL×1
Microplate (Coated)	12 wells×8 strips
Sample diluent	6mL×1
Chromogen substrate A	6mL×1
Chromogen substrate B	6mL×1
Stop solution	6mL×1
Positive control	0.5mL×1
Negative control	0.5mL×1

Description

This kit uses the double antigen sandwich method to determine the expression of human hepatitis C virus antibodies (HCV Ab) in specimens. Using purified antigen coated microplates to prepare solid-phase antigens, they can bind to hepatitis C virus antibodies (HCV Ab) in the sample. After washing to remove unbound antibodies and other components, they can then bind to HRP labeled antigens to form antigen antibody enzyme labeled antigen complexes. After thorough washing, TMB substrate is added for color development. TMB is converted to blue under the catalysis of HRP enzyme and to the final yellow under the action of acid. Measure the absorbance (OD value) at 450nm wavelength using an enzyme-linked immunosorbent assay (ELISA) reader and compare it with the CUTOFF value to determine the presence or absence of human hepatitis C virus antibodies (HCV Ab) in the specimen.

Application

This kit is used to determine the expression of hepatitis C virus antibodies (HCV Ab) in human serum, plasma, and related liquid samples.

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