Manual

Product Number: ELK059

Shipping and Storage

1. Reagent kit storage: 2-8℃.
2. Validity period: 6 months.

Component

Component	48T
Microplate	8 wells×6 strips
Calibration standard (10 ×)	100μL
Enzyme marker (100 ×)	50μL
Universal diluent  (20 ×)	15mL
Concentrated washing solution (20 ×)	25mL
Chromogen substrate	12mL
Stop solution	6mL

If you need to purchase universal components separately, please provide the corresponding component names.

Description

The reagent kit adopts the principle of double antibody sandwich method: the capture antibody is wrapped on the enzyme-linked immunosorbent assay (ELISA) plate, and the analyte in the sample and calibration sample is captured. The enzyme-linked secondary antibody labeled with horseradish peroxidase is added to form an "antibody antigen antibody HRP" immune complex. After adding TMB for color development, if the analyte in the sample appears blue, the reaction is stopped by adding termination solution. During the detection process, free components are washed away, and the OD value is measured at 450 nm using an enzyme-linked immunosorbent assay (ELISA) reader. The color intensity is proportional to the content of the analyte in the sample, and the concentration of the analyte in the sample is calculated by plotting a standard curve.

Application

This kit is used to detect the concentration of human natural killer (NK) antigen in serum, plasma and other samples for research purposes only.

Testing Scope

0.312-20ng/mL

---