Manual

Product Number: ELK21812

Shipping and Storage

1. Reagent kit storage: 2-8℃.
2. Validity period: 6 months.

Component

Description

This kit uses the double antibody sandwich method to determine the expression of human hepatitis E antigen (HEV) in specimens. Purified human hepatitis E antigen (HEV) antibody is coated onto a microplate to prepare a solid-phase antibody that can bind to hepatitis E antigen (HEV) in the sample. After washing to remove unbound antigens and other components, it binds to HRP labeled hepatitis E antigen (HEV) antibody to form an antibody antigen enzyme-linked antibody complex. After thorough washing, TMB substrate is added for color development. TMB is converted to blue under the catalysis of HRP enzyme and to the final yellow under the action of acid. Measure the absorbance (OD value) at 450nm wavelength using an enzyme-linked immunosorbent assay (ELISA) reader and compare it with the CUTOFF value to determine the presence or absence of human hepatitis E antigen (HEV) in the specimen.

Application

This kit is used to determine the expression of hepatitis E antigen (HEV) in human serum, plasma, and related liquid samples.

Specimen requirements

1. Extract the specimen as soon as possible after collection, according to relevant literature, and conduct experiments as soon as possible after extraction. If the experiment cannot be conducted immediately, the specimen can be stored at -20℃, but repeated freezing and thawing should be avoided.
2. Samples containing NaN3 cannot be detected because NaN3 inhibits the activity of horseradish peroxidase (HRP).

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