Human Immunoglobulin E, IgE ELISA Kit
2026-05-29Human Immunoglobulin G, IgG ELISA Kit
2026-05-29Human Immunoglobulin M, IgM ELISA Kit
Product Number: ELK11522
Shipping and Storage
- Reagent kit storage: 2-8℃.
- Validity period: 6 months.
Component
| Component | 96T |
| 30× concentrated wash buffer | 20mL×1 |
| Enzyme conjugate | 6mL×1 |
| Microplate (Coated) | 12 wells×8 strips |
| Sample diluent | 6mL×1 |
| Chromogen substrate A | 6mL×1 |
| Chromogen substrate B | 6mL×1 |
| Stop solution | 6mL×1 |
| Standard (24μg/mL) | 0.5mL×1 |
| Standard diluent | 1.5mL×1 |
Description
This kit uses the double antigen sandwich method to determine the level of human immunoglobulin M (IgM) in specimens. Coat the microporous plate with purified antigen to prepare solid-phase antigen. Add immunoglobulin M (IgM) to the coated micropores in sequence, and then bind with HRP labeled antigen to form an antigen antibody enzyme labeled antigen complex. After thorough washing, add substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and to the final yellow under the action of acid. The depth of color is positively correlated with immunoglobulin M (IgM) in the sample. Measure the absorbance (OD value) at 450nm wavelength using an enzyme-linked immunosorbent assay (ELISA) reader, and calculate the concentration of human immunoglobulin M (IgM) in the sample using a standard curve.
Application
This kit is used to determine the content of immunoglobulin M (IgM) in human serum, plasma, and related liquid samples.
Testing Scope
0.3μg/mL -14μg/mL
