Bovine Theileria annulata ELISA Kit (Qualitative)
2026-05-29
Diagnostic Kit for Pseudomonas Aeruginosa (PA) DNA
2026-06-10Human TNF-α ELISA Kit
Product Number: ELK10110
Shipping and Storage
- Reagent kit storage: 2-8℃.
- Validity period: 6 months.
Component
| Component | 96T |
| 30× concentrated wash buffer | 20mL×1 |
| Enzyme conjugate | 6mL×1 |
| Microplate (Coated) | 12 wells×8 strips |
| Sample diluent | 6mL×1 |
| Chromogen substrate A | 6mL×1 |
| Chromogen substrate B | 6mL×1 |
| Stop solution | 6mL×1 |
| Standard (800ng/L) | 0.5mL×1 |
| Standard diluent | 1.5mL×1 |
Description
This kit uses a double antibody sandwich method to determine the level of human tumor necrosis factor alpha (TNF - α) in specimens. Coat a microplate with purified human tumor necrosis factor alpha (TNF - α) antibody to prepare a solid-phase antibody. Add tumor necrosis factor alpha (TNF - α) to the coated micropores in sequence, and then bind with HRP labeled tumor necrosis factor alpha (TNF - α) antibody to form an antibody antigen enzyme labeled antibody complex. After thorough washing, add substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and to the final yellow under the action of acid. The depth of color is positively correlated with tumor necrosis factor alpha (TNF - α) in the sample. Measure the absorbance (OD value) at 450nm wavelength using an enzyme-linked immunosorbent assay (ELISA) reader, and calculate the concentration of human tumor necrosis factor alpha (TNF - α) in the sample using a standard curve.
Application
This kit is used to determine the content of tumor necrosis factor alpha (TNF - α) in human serum, plasma, cell supernatant, and related liquid samples.
Testing Scope
20ng/L -400ng/L
