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2026-08-31Human Soluble Vascular Cell Adhesion Molecule-1 sVCAM-1 ELISA Kit (Quantitative)
Product Number: ELK12J1009
Shipping and Storage
Store at 2-8℃, away from light and moisture. Validity period: 6 months.
Component
| Component | 96-Well Configuration | 48-Well Configuration |
| Microplate | 8 wells×12 strips | 8 wells×6 strips |
| Standards | 0.3mL×6 pipes | 0.3mL×6 pipes |
| Sample diluent | 6mL | 3mL |
| Detection Antibody-HRP | 10mL | 5mL |
| 20×washing buffer | 25mL | 15mL |
| Substrate A | 6mL | 3mL |
| Substrate B | 6mL | 3mL |
| Stop solution | 6mL | 3mL |
| Plate Sealer | 2 sheets | 2 sheets |
Note: The concentrations of standard samples (S0-S5) are 0、3.125、6.25、12.5、25、50ng/mL, respectively.
Description
The reagent kit adopts a double antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). Add the specimen, standard, and HRP labeled detection antibody in sequence to the pre coated micropores with human vascular endothelial cell adhesion molecule-1 (VCAM-1) antibody, incubate and thoroughly wash. Using substrate TMB for color development, TMB is converted to blue under the catalysis of peroxidase and to the final yellow under the action of acid. The depth of color is positively correlated with human vascular endothelial cell adhesion molecule-1 (VCAM-1) in the sample. Measure the absorbance (OD value) at a wavelength of 450nm using an enzyme-linked immunosorbent assay (ELISA) reader and calculate the sample concentration.
