Human Immunodeficiency Virus Type 1 (HIV-I) Nucleic Acid Detection Kit (Fluorescent PCR Method)
2025-04-07
Blue LED Transilluminator
2025-04-07Human Hepatitis B surface Antibody IgG Quantitative Eilsa Kit
Product Number: HBA01
Shipping and Storage
- 2-8℃, stored away from light and moisture
- Validity period: 6 months
Component
| Component | 96T | 48T |
| Microporous enzyme-linked immunosorbent assay (ELISA) plate | 12 holes×8 strips | 12 holes×4 strips |
| negative control | 0.5mL | 0.5mL |
| positive control | 0.5mL | 0.5mL |
| Detect antibody HRP | 10mL | 5mL |
| 20× washing buffer solution | 25mL | 15mL |
| Sample diluent | 6mL | 3mL |
| Substrate A | 6mL | 3mL |
| Substrate B | 6mL | 3mL |
| Stop solution | 6mL | 3mL |
| Microplate Sealers | 2 sheets | 2 sheets |
Description
The reagent kit adopts indirect enzyme-linked immunosorbent assay (ELISA). Add the sample and HRP labeled detection antibody to the pre coated micropores of hepatitis B virus surface antigen, incubate and thoroughly wash. Using substrate TMB for color development, TMB is converted to blue under the catalysis of peroxidase and to the final yellow under the action of acid. Measure the absorbance (OD value) at a wavelength of 450nm using an enzyme-linked immunosorbent assay (ELISA) reader, and compare it with the CUTOFF value to determine the presence or absence of hepatitis B virus surface IgG antibodies (HBs IgG) in the specimen.
Specimen collection and storage
- Serum: Use test tubes without pyrogen and endotoxin, avoid any cell irritation during the operation, collect blood, centrifuge at 3000 rpm for 10 minutes, and quickly and carefully separate serum and red blood cells.
- Plasma: EDTA, Citrate or heparin anticoagulant. Centrifuge at 3000 rpm for 30 minutes and collect the supernatant.
- Cell supernatant: Centrifuge at 3000 rpm for 10 minutes to remove particles and polymers.
- Tissue homogenate: Crush the tissue by adding an appropriate amount of physiological saline. Centrifuge at 3000 rpm for 10 minutes and collect the supernatant.
Materials required but not supplied
- ELISA reader (450nm)
- High precision sampler and nozzle: 0.5-10μL, 2-20μL, 20-200μL, 200-1000μL.
- 37℃ constant temperature box.
