20×Sybr Green I
2025-08-06
Blood Agar Base
2025-08-07Product Number: RNK4501LY
Shipping and Storage
Freeze dried powder is stored at 4 ℃, and after reconstitution, the solution is stored at -20 ℃
Components
| Component | RNK4501LY |
| DNase I ,lyophilized powder | 1 |
| Reaction Buffer(with MgCl2),10× | 1mL |
| 200 mM EDTA | 1mL |
| RNase Free Water | 2×1mL |
Description
DNase I is a deoxyribonuclease that requires a divalent cation and can be used to degrade single stranded or double stranded DNA. The principle is that DNase I hydrolyzes phosphate diester bonds to produce single nucleotides or oligonucleotides with 5 '- phosphate groups and 3' - OH groups. Both Mg2+ and Mn2+ can activate the activity of DNase I, while Ca2+ concentration directly affects enzyme activity. When Mg2+ is present, it can randomly generate incisions on each single strand of double stranded DNA; In the presence of Mn2+, double stranded DNA can be broken and fragmented. Used for the preparation of RNA without DNA contamination, reverse transcription, and in vitro transcription experiments.
