Manual

Product Number: ELK054

Shipping and Storage

1. Reagent kit storage: 2-8℃.
2. Validity period: 6 months.

Component

Component	48T
20× concentrated wash buffer	15mL×1
Enzyme conjugate	6mL×1
Microplate (Coated)	8 wells×6 strips
Sample diluent	10mL×1
Chromogen substrate	6mL×1
Stop solution	3mL×1
Positive control	0.5mL×1
Negative control	0.5mL×1

Description

This kit uses the double antibody sandwich method to determine the expression of human Newcastle disease virus (NDV) in specimens. Using purified Newcastle disease virus (NDV) antibodies coated on microplates, solid-phase antibodies can be prepared that can bind to Newcastle disease virus (NDV) in the sample. After washing to remove unbound antigens and other components, they can then bind to HRP labeled antibodies to form antibody antigen enzyme labeled antibody complexes. After thorough washing, TMB substrate is added for color development. TMB is converted to blue under the catalysis of HRP enzyme and to the final yellow under the action of acid. Measure the absorbance (OD value) at a wavelength of 450nm using an enzyme-linked immunosorbent assay (ELISA) reader, and compare it with the CUTOFF value to determine the presence or absence of human Newcastle disease virus (NDV) in the specimen.

Application

This kit is used to determine the expression of Newcastle disease virus (NDV) in human serum, plasma, cells, tissues, and related liquid samples.

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