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2026-04-28Aldolase (ALS) Activity Assay Kit
Product Number: BC2275
Shipping and Storage
Store at 2-8℃.
Component
| Component | 100T |
| R1(Buffer/Substrate) | Powder × 5 bottles |
| R2(NADH) | Powder × 2 bottles |
| R3(GDH/TIM/LDH) | Liquid × 1 bottle |
Solution preparation: R1: Add 20mL of distilled water to each bottle before use, and store at 2-8℃ for 2 weeks; R2: Dissolve 1mL of distilled water in each bottle before use and store at 2-8℃ for 4 weeks; R3: Can be used directly.
Description
Fructose 1,6-diphosphate aldolase (FBA) (EC. 4.1.2.13) is an important enzyme involved in the calvin cycle in glycolysis, gluconeogenesis, pentose phosphate pathway, and photosynthesis. It catalyzes the reversible cleavage of fructose 1,6-diphosphate into phosphodihydroxyacetone and 3-phosphoglyceraldehyde, and is widely present in animals, plants, and microorganisms, exhibiting different responses under various stress conditions.
Fructose 1,6-diphosphate aldolase catalyzes the conversion of fructose 1,6-diphosphate (F-1,6-DP) to 3-phosphoglyceraldehyde (GAP) and phosphodihydroxyacetone (DAP), and catalyzes the conversion of NADH and phosphodihydroxyacetone to NAD and α - phosphoglycerol under the action of phosphoglucose isomerase and α - phosphoglycerol dehydrogenase. The change in absorbance at 340nm can reflect the activity of fructose 1,6-diphosphate aldolase.
