Polybrene (10 mg/mL)
2026-08-18
Fructose-1, 6-bisphosphate aldolase (FBA) Activity Assay Kit (Microplate method)
2026-08-21Fructose-1, 6-bisphosphate aldolase (FBA) Activity Assay Kit (UV method)
Product Number: BC2271
Shipping and Storage
- Reagent 1 and Reagent 2 : Store at -20℃;
- Reagent 3: Store at -20℃ away from light;
- Extraction Solution, Reagent 4 and Reagent 5: Store at 4℃.
Component
| Component | 48T |
| Extraction Solution | 60mL× 1 bottle |
| Reagent 1 | Liquid, 1 bottle |
| Reagent 2 | Powder, 4 vials |
| Reagent 3 | Liquid, 1 bottle |
| Reagent 4 | 30mL × 1 bottle |
| Reagent 5 | Powder × 1 bottle |
- Reagent 1: Tap or centrifuge the vial before opening to collect all reagent at the bottom; Add 2.1mL of distilled water for reconstitution prior to use; Valid for the same shelf‑life as the kit.
- Reagent 2: For each vial: Centrifuge at 8000 g, 4℃ for 2 min before use to pellet powder to the tube bottom. Add 0.6mL distilled water to dissolve. Aliquot unused portions and store at −20℃. Avoid repeated freeze‑thaw cycles. Use within 3 days after reconstitution.
- Reagent 3: Tap or centrifuge the vial before opening to collect all reagent at the bottom. Add 2.2mL distilled water for reconstitution prior to use. Valid for the same shelf‑life as the kit.
- Reagent 5: Tap or centrifuge the vial before opening to collect all powder at the bottom. Add 4.2mL distilled water for reconstitution prior to use. Valid for the same shelf‑life as the kit.
Description
Fructose‑1,6‑bisphosphate aldolase (EC 4.1.2.13, FBA) participates in glycolysis/gluconeogenesis as well as the pentose phosphate cycle. It supplies ATP energy and substrates for biosynthetic metabolism in organisms, and therefore plays a critical role in cellular vital activities.
FBA catalyzes the cleavage of fructose‑1,6‑bisphosphate into glyceraldehyde‑3‑phosphate and dihydroxyacetone phosphate. Under sequential action of coupled enzymatic complexes, NADH and dihydroxyacetone phosphate are converted into NAD⁺ and α‑glycerophosphate. FBA activity is determined by monitoring the decreasing rate of NADH absorbance at 340nm.
