Glucose-6-phosphate dehydrogenase (G6PDH) kit (visible colorimetric method), spectrophotometry
2026-08-07Glucose-6-phosphate dehydrogenase (G6PDH) kit (visible colorimetric method), Microplate method
Product Number: G6PDH002
Shipping and Storage
Valid for 3 months, different reagents have different storage temperatures, please refer to the actual reagent label for storage.
Component
| Component | 96T | Storage |
| Extraction Buffer | 100mL × 1 | 4℃ |
| Reagent 1 | 25mL × 1 | 4℃ |
| Reagent 2 | Powder, 1 bottle | 4℃ |
| Reagent 3 | 1.1mL × EP tube | 4℃, protected from light |
| Standard | Powder, 1 vial | -20℃ |
Note: Reagent 2: Tap the bottle gently to settle all powder to the bottom before opening; Add 19mL of Reagent 1 to dissolve thoroughly. Unused reagent shall be stored at 4°C.
Standard: This reagent is used when a new standard curve needs to be prepared; Prepare according to the standard curve preparation steps in the instruction; Use up the dissolved standard within one week.
Description
Glucose-6-phosphate dehydrogenase (G6PDH; EC 1.1.1.49) is widely present in animals, plants, microorganisms and cultured cells. It is a key enzyme in the pentose phosphate pathway, and maintains the intracellular level of NADPH. NADPH in turn maintains the glutathione level in cells, thereby protecting cells from oxidative damage. Therefore, the activity of G6PDH can reflect the biosynthesis and antioxidant capacity of organisms to a certain extent.
G6PDH catalyzes the oxidation of glucose-6-phosphate to 6-phosphogluconolactone, and simultaneously reduces NADP⁺ to NADPH. The traditional method detects the absorbance of NADPH at 340nm. Due to the low molar extinction coefficient (ε) of NADPH, this method has low sensitivity and is severely susceptible to interference.
This kit provides a simple, sensitive and rapid assay method: the NADPH produced by this enzymatic process reacts with a specific chromogenic reagent to produce a colored substance with a maximum absorption peak at 450nm. The G6PDH enzyme activity is calculated by detecting the increase rate of absorbance at 450nm.
