Supercompetent Cell Preparation Kit
2026-08-28
Human Soluble Toll-Like Receptor 2 (sTLR-2) ELISA Kit (Quantitative)
2026-08-31Human Cotinine ELISA Kit (Quantitative)
Product Number: ELK02J1226
Shipping and Storage
Store at 2-8℃, away from light and moisture. Validity period: 6 months.
Component
| Component | 96-Well Configuration | 48-Well Configuration |
| Microplate | 8 wells×12 strips | 8 wells×6 strips |
| Standards | 0.3mL×6 pipes | 0.3mL×6 pipes |
| Sample diluent | 6mL | 3mL |
| Detection Antibody-HRP | 6mL | 3mL |
| 20×washing buffer | 25mL | 15mL |
| Substrate A | 6mL | 3mL |
| Substrate B | 6mL | 3mL |
| Stop solution | 6mL | 3mL |
| Plate Sealer | 2 sheets | 2 sheets |
Note: The concentrations of standard samples (S0-S5) are 0、3.125、6.25、12.5、25、50ng/mL, respectively.
Description
The reagent kit adopts competitive enzyme-linked immunosorbent assay (ELISA). Add the sample, standard, and HRP labeled competitive antigen to the pre coated micropores with cotinine antibody, incubate and thoroughly wash. Using substrate TMB for color development, TMB is converted to blue under the catalysis of peroxidase and to the final yellow under the action of acid. The depth of color is negatively correlated with cotinine in the sample. Measure the absorbance (OD value) at a wavelength of 450nm using an enzyme-linked immunosorbent assay (ELISA) reader and calculate the sample concentration.
