Bacillus cereus (BC) Nucleic Acid Detection Kit (Fluorescent PCR Method)
2026-10-26
Human Transforming growth factor beta 1 (TGF-β1)
2026-10-26Rat Catalase (CAT)
Product Number: ELK02J1118
Shipping and Storage
Store at 2-8℃, away from light and moisture. Validity period: 6 months.
Component
| Component | 96-Well Configuration | 48-Well Configuration |
| Microplate | 8 wells×12 strips | 8 wells×6 strips |
| Standards | 0.3mL×6 pipes | 0.3mL×6 pipes |
| Sample diluent | 6mL | 3mL |
| Detection Antibody-HRP | 10mL | 5mL |
| 20×washing buffer | 25mL | 15mL |
| Substrate A | 6mL | 3mL |
| Substrate B | 6mL | 3mL |
| Stop solution | 6mL | 3mL |
| Plate Sealer | 2 sheets | 2 sheets |
Note: The concentrations of standard samples (S0-S5) are 0、62.5、125、250、500、1000pg/mL, respectively.
Description
The kit adopts the one-step double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Add samples, standards and HRP-labeled detection antibody sequentially into the microplate wells pre-coated with rat catalase (CAT) antibody, followed by incubation and thorough washing. Color development is performed with TMB substrate. TMB is converted to blue under the catalysis of peroxidase and finally turns yellow under acidic conditions. The color intensity is positively correlated with the concentration of rat catalase (CAT) in the sample. Measure the absorbance (OD value) at a wavelength of 450nm using a microplate reader to calculate the sample concentration.
