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Product Number: BTA02
Shipping and Storage
- 2-8℃, stored away from light and moisture
- Validity period: 6 months
Component
| Component | 96T | 48T |
| Microplate (96-well, pre-coated) | 12 holes×8 strips | 12 holes×4 strips |
| Standard Sample | 0.3mL×6 tubes | 0.3mL×6 tubes |
| Sample diluent | 6mL | 3mL |
| HRP-Conjugated Detection Antibody | 10mL | 5mL |
| 20× washing buffer | 25mL | 15mL |
| TMB Substrate A | 6mL | 3mL |
| TMB Substrate B | 6mL | 3mL |
| Stop solution | 6mL | 3mL |
| Plate Sealer | 2 sheets | 2 sheets |
Note: The concentrations of standard samples (S0-S5) are 0, 50, 100, 200, 400, and 800 pg/mL, respectively.
Description
The reagent kit adopts a double antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). Add the specimen, standard, and HRP labeled detection antibody to the pre coated micropores containing the circular Taylor's Charcot worm antigen, incubate and thoroughly wash. Using substrate TMB for color development, TMB is converted to blue under the catalysis of peroxidase and to the final yellow under the action of acid. The depth of color is positively correlated with Theileria Annulata Ab antibodies in the sample. Measure the absorbance (OD value) at a wavelength of 450nm using an enzyme-linked immunosorbent assay (ELISA) reader and calculate the sample concentration.
