Human Methyltransferase-like 3 (METTL3) (Quantitative)
2026-09-14Human ferredoxin 1 (FDX1) ELISA Kit (Quantitative)
2026-09-14Human Podoplanin (PDPN) ELISA Kit (Quantitative)
Product Number: ELK12J1014
Shipping and Storage
Store at 2-8℃, away from light and moisture. Validity period: 6 months.
Component
| Component | 96-Well Configuration | 48-Well Configuration |
| Microplate | 8 wells×12 strips | 8 wells×6 strips |
| Standards | 0.3mL×6 pipes | 0.3mL×6 pipes |
| Sample diluent | 6mL | 3mL |
| Detection Antibody-HRP | 10mL | 5mL |
| 20×washing buffer | 25mL | 15mL |
| Substrate A | 6mL | 3mL |
| Substrate B | 6mL | 3mL |
| Stop solution | 6mL | 3mL |
| Plate Sealer | 2 sheets | 2 sheets |
Note: The concentrations of standard samples (S0-S5) are 0、100、200、400、800、1600pg/mL, respectively.
Description
The reagent kit adopts a double antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). Add the sample, standard, and HRP labeled detection antibody in sequence to the pre coated micropores with PDPN antibody, incubate and thoroughly wash. Using substrate TMB for color development, TMB is converted to blue under the catalysis of peroxidase and to the final yellow under the action of acid. The depth of color is positively correlated with human podocyte protein (PDPN) in the sample. Measure the absorbance (OD value) at a wavelength of 450nm using an enzyme-linked immunosorbent assay (ELISA) reader and calculate the sample concentration.
